Microsatellite Markers for the New Zealand Endemic Tree <i>Fuchsia excortica</i>ta (Onagraceae)

Abstract

Premise of the study: Microsatellite markers were developed from a New Zealand endemic understory tree, Fuchsia excorticata, to investigate factors affecting the mating system. Methods and Results: Using 454 pyrosequencing, 48 microsatellite markers were developed and tested for polymorphism within populations. Twelve of these microsatellite loci were identified as being polymorphic within at least three populations and consistently amplified in the four populations tested. These primers amplified di-, tri-, and tetranucleotide repeats with 1–10 alleles per population. Conclusions: These results indicate the utility of microsatellite loci for future mating system and population genetic studies in F. excorticata.

Keywords

• Fuchsia excorticata
• microsatellite markers
• Onagraceae
• shotgun 454 pyrosequencing