In-situ enrichment of ARGs and their carriers in soil by hydroxamate siderophore: A promising biocontrol approach for source reduction

Shengwei Yi; Ming Wei; Feng Li; Xingang Liu; Qingqing Fan; Hainan Lu; Yujun Wu; Yun Liu; Jiang Tian; Ming Zhang

Environment International (Aug 2024)

In-situ enrichment of ARGs and their carriers in soil by hydroxamate siderophore: A promising biocontrol approach for source reduction

Shengwei Yi,
Ming Wei,
Feng Li,
Xingang Liu,
Qingqing Fan,
Hainan Lu,
Yujun Wu,
Yun Liu,
Jiang Tian,
Ming Zhang

Affiliations

Shengwei Yi: College of Environment and Resources, Xiangtan University, Xiangtan 411105, China; Hunan Provincial University Key Laboratory for Environmental and Ecological Health, Xiangtan University, Xiangtan 411105, China; Hunan Provincial University Key Laboratory for Environmental Behavior and Control Principle of New Pollutants, Xiangtan University, Xiangtan 411105, China
Ming Wei: College of Environment and Resources, Xiangtan University, Xiangtan 411105, China; Hunan Provincial University Key Laboratory for Environmental and Ecological Health, Xiangtan University, Xiangtan 411105, China; Hunan Provincial University Key Laboratory for Environmental Behavior and Control Principle of New Pollutants, Xiangtan University, Xiangtan 411105, China
Feng Li: College of Environment and Resources, Xiangtan University, Xiangtan 411105, China; Hunan Provincial University Key Laboratory for Environmental and Ecological Health, Xiangtan University, Xiangtan 411105, China; Hunan Provincial University Key Laboratory for Environmental Behavior and Control Principle of New Pollutants, Xiangtan University, Xiangtan 411105, China; Corresponding author at: College of Environment and Resources, Xiangtan University, Xiangtan 411105, China.
Xingang Liu: College of Environment and Resources, Xiangtan University, Xiangtan 411105, China; Hunan Provincial University Key Laboratory for Environmental and Ecological Health, Xiangtan University, Xiangtan 411105, China; Hunan Provincial University Key Laboratory for Environmental Behavior and Control Principle of New Pollutants, Xiangtan University, Xiangtan 411105, China
Qingqing Fan: College of Environment and Resources, Xiangtan University, Xiangtan 411105, China; Hunan Provincial University Key Laboratory for Environmental and Ecological Health, Xiangtan University, Xiangtan 411105, China; Hunan Provincial University Key Laboratory for Environmental Behavior and Control Principle of New Pollutants, Xiangtan University, Xiangtan 411105, China
Hainan Lu: Shanghai Academy of Environmental Sciences, Shanghai 200233, China
Yujun Wu: College of Environment and Resources, Xiangtan University, Xiangtan 411105, China; Hunan Provincial University Key Laboratory for Environmental and Ecological Health, Xiangtan University, Xiangtan 411105, China; Hunan Provincial University Key Laboratory for Environmental Behavior and Control Principle of New Pollutants, Xiangtan University, Xiangtan 411105, China
Yun Liu: College of Environment and Resources, Xiangtan University, Xiangtan 411105, China; Hunan Provincial University Key Laboratory for Environmental and Ecological Health, Xiangtan University, Xiangtan 411105, China; Hunan Provincial University Key Laboratory for Environmental Behavior and Control Principle of New Pollutants, Xiangtan University, Xiangtan 411105, China
Jiang Tian: College of Environment and Resources, Xiangtan University, Xiangtan 411105, China; Hunan Provincial University Key Laboratory for Environmental and Ecological Health, Xiangtan University, Xiangtan 411105, China; Hunan Provincial University Key Laboratory for Environmental Behavior and Control Principle of New Pollutants, Xiangtan University, Xiangtan 411105, China
Ming Zhang: Department of Environmental Engineering, China Jiliang University, Hangzhou 310018, China

Journal volume & issue: Vol. 190
p. 108915

Abstract

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Pathogenic microorganisms with antibiotic resistance genes (ARGs) pose a serious threat to public health and soil ecology. Although new drugs and available antibacterial materials can kill ARG carriers but accidentally kill beneficial microorganisms. Therefore, the rapid enrichment and separation of ARGs and their carriers from soil is becoming an important strategy for controlling the diffusion of ARGs. Hydroxamate siderophore (HDS) has gained widespread attentions for its involvement in trace element transfer among microorganisms in the soil environment, we thus explored an in-situ trapping-enrichment method for ARGs and their carriers via a small molecular HDS secreted by Pseudomonas fluorescens HMP01. In this study, we demonstrate that HDS significantly in-situ traps and enriches certain ARGs, including chloramphenicol, MLS, rifamycin, and tetracycline resistance genes in the soil environment. The enrichment efficiencies were 1473-fold, 38-fold, 17-fold, and 5-fold, respectively, higher than those in the control group. Specifically, the primary enriched ARGs were rpoB, mphL, catB2, and tetA(60), and Bacillus, Rhizobium, Rossellomorea, and Agrobacterium were hosts for these ARGs. This enrichment was caused by the upregulation of chemotaxis genes (e.g., cheW, cheC, and cheD) and rapid biofilm formation within the enriched bacterial population. Notably, representative ARGs such as cat, macB, and rpoB were significantly reduced by 36%, 85.7%, and 72%, respectively, in the paddy soil after HDS enrichment. Our research sheds light on the potential application of siderophore as a rapping agent for the eco-friendly reduction of ARGs and their carriers in soil environments.

Published in Environment International

ISSN: 0160-4120 (Print)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Geography. Anthropology. Recreation: Environmental sciences
Website: https://www.journals.elsevier.com/environment-international

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