There is a few reported about the volatile oil component of petal, herbal and component of seed oil of borage. This research worked carried out for analysis and identification the volatile oil in herbals, petals, and seed oil compositions of Borago officinalis L. in Lorestan province. Material and methods: Extraction of essential oil from petals carried out using steam distillation by Clevenger apparatus. The new SPME-GC/MS method is used for extraction and identification of volatile oil compounds in the herbal of borage. The oil of the seeds was extracted using a Cold-press method. The identification of chemical composition of extracted oil was carried out by GC/MS apparatus. Results: In petals of Borage only Carvacerol component, and in the herbal of Borage three components Carvacrol, Bisabolone oxide and 2-Phenylethyl benzoate, extracted and identified respectively. In the seed oil of borage 16 different components were separated and identified. The following components had the highest amount in seed oil: Hexadecane, N, N-dimethylethanolamine, Beta-d-glycoside, 3, 6-glucurono-methyl, Benzaldehde, 4-methyl 3-Hydroxytetrahydrofuran, Hexadecanoic acid, Heptanoic acid, Gamma butyrolactone and Ethyl octadec-9-enoate are the major components respectively. These components contain 63.4% of all components in borage seed oil and the 7 residual components only 9.5% all of the components in borage seed oil. Also one unknown (27.1%) component identified. Conclusion: Using result obtained from this research the volatile oil a few amounts of the borage chemical composition. The results show that the seed oil of this species can be used for medicinal preparation. Cold Press method was found to be rapid and simple for identification of seeds oil components.