Research Note: Identification and characterization of Salmonella spp. in mechanically deboned chickens using pulsed-field gel electrophoresis

Angela Jie-won Ha; Lurdes G. Siberio Perez; Tae-Jo Kim; Md. Furkanur Rahaman Mizan; Shamsun Nahar; Si-Hong Park; Hyang-Sook Chun; Sang-Do Ha

Poultry Science (Mar 2021)

Research Note: Identification and characterization of Salmonella spp. in mechanically deboned chickens using pulsed-field gel electrophoresis

Angela Jie-won Ha,
Lurdes G. Siberio Perez,
Tae-Jo Kim,
Md. Furkanur Rahaman Mizan,
Shamsun Nahar,
Si-Hong Park,
Hyang-Sook Chun,
Sang-Do Ha

Affiliations

Angela Jie-won Ha: Department of Food Science, Nutrition, and Health Promotion, Mississippi State University, Starkville, USA; Department of Food Science and Technology, Advanced Food Safety Research Group, BrainKorea21 Plus, Chung-Ang University, Korea
Lurdes G. Siberio Perez: Department of Food Science, Nutrition, and Health Promotion, Mississippi State University, Starkville, USA
Tae-Jo Kim: Department of Food and Nutrition, University of Wisconsin-Stout, Menomonie, USA
Md. Furkanur Rahaman Mizan: Department of Food Science and Technology, Advanced Food Safety Research Group, BrainKorea21 Plus, Chung-Ang University, Korea
Shamsun Nahar: Department of Food Science and Technology, Advanced Food Safety Research Group, BrainKorea21 Plus, Chung-Ang University, Korea
Si-Hong Park: Department of Food Science & Technology, Oregon State University, Corvallis, USA
Hyang-Sook Chun: Department of Food Science and Technology, Advanced Food Safety Research Group, BrainKorea21 Plus, Chung-Ang University, Korea
Sang-Do Ha: Department of Food Science and Technology, Advanced Food Safety Research Group, BrainKorea21 Plus, Chung-Ang University, Korea; Corresponding author:

Journal volume & issue: Vol. 100, no. 3
p. 100961

Abstract

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Salmonella is one of the common foodborne bacteria, causing 80.3 million illnesses every year worldwide. This study was conducted to isolate and identify Salmonella enterica serovars from poultry samples responsible for causing foodborne poisoning in the Mississippi area, United States. A total of 55 S. enterica serovars—Enteritidis (6), Oranienburg (1), Schwarzengrund (8), Heidelberg (4), Kentucky (22), 4, [5], 12:i:- (1), Montevideo (2), Infantis (9), and multi serotypes (2)—were isolated from approximately 110 poultry samples. Through pulsed-field gel electrophoresis (PFGE) analysis, 8 to 13 bands were obtained. The profiles showed >90% similarity in strains within the same type. Consequently, PFGE could be a useful tool to determine chromosomal similarity (clonality of strains) that can be used to trace down epidemiologic sources and geographical origins of Salmonella.

Published in Poultry Science

ISSN: 0032-5791 (Print); 1525-3171 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Agriculture: Animal culture
Website: https://www.journals.elsevier.com/poultry-science

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