Single nucleotide polymorphisms (SNPs) in the open reading frame (ORF) of prion protein gene (PRNP) in Nigerian livestock species
Adeniyi C. Adeola,
Semiu F. Bello,
Abdussamad M. Abdussamad,
Rahamon A. M. Adedokun,
Sunday C. Olaogun,
Nasiru Abdullahi,
Akanbi I. Mark,
Anyebe B. Onoja,
Oscar J. Sanke,
Godwin F. Mangbon,
Jebi Ibrahim,
Philip M. Dawuda,
Adebowale E. Salako,
Samia Kdidi,
Mohamed Habib Yahyaoui
Affiliations
Adeniyi C. Adeola
State Key Laboratory of Genetic Resources and Evolution & Yunnan Laboratory of Molecular Biology of Domestic Animals, Kunming Institute of Zoology, Chinese Academy of Sciences
Semiu F. Bello
Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University
Abdussamad M. Abdussamad
Department of Veterinary Physiology and Biochemistry, Faculty of Veterinary Medicine, Bayero University
Rahamon A. M. Adedokun
Department of Veterinary Medicine, Faculty of Veterinary Medicine, University of Ibadan
Sunday C. Olaogun
Department of Veterinary Medicine, Faculty of Veterinary Medicine, University of Ibadan
Nasiru Abdullahi
Department of Biochemistry, Faculty of Basic Medical Sciences, College of Health Sciences, Bayero University
Akanbi I. Mark
Ministry of Agriculture and Rural Development
Anyebe B. Onoja
Department of Virology, College of Medicine, University of Ibadan
Oscar J. Sanke
Taraba State Ministry of Agriculture and Natural Resources
Godwin F. Mangbon
Division of Veterinary Office
Jebi Ibrahim
Department of Veterinary Surgery and Theriogenology, College of Veterinary Medicine, University of Agriculture Makurdi
Philip M. Dawuda
Department of Animal Science, Faculty of Agriculture, National University of Lesotho
Adebowale E. Salako
Department of Animal Science, Faculty of Agriculture, University of Ibadan
Samia Kdidi
Livestock and Wildlife Laboratory, Institut des Régions Arides, Université de Gabes
Mohamed Habib Yahyaoui
Livestock and Wildlife Laboratory, Institut des Régions Arides, Université de Gabes
Abstract Background Prion diseases, also known as transmissible spongiform encephalopathies (TSEs) remain one of the deleterious disorders, which have affected several animal species. Polymorphism of the prion protein (PRNP) gene majorly determines the susceptibility of animals to TSEs. However, only limited studies have examined the variation in PRNP gene in different Nigerian livestock species. Thus, this study aimed to identify the polymorphism of PRNP gene in Nigerian livestock species (including camel, dog, horse, goat, and sheep). We sequenced the open reading frame (ORF) of 65 camels, 31 village dogs and 12 horses from Nigeria and compared with PRNP sequences of 886 individuals retrieved from public databases. Results All the 994 individuals were assigned into 162 haplotypes. The sheep had the highest number of haplotypes (n = 54), and the camel had the lowest (n = 7). Phylogenetic tree further confirmed clustering of Nigerian individuals into their various species. We detected five non-synonymous SNPs of PRNP comprising of G9A, G10A, C11G, G12C, and T669C shared by all Nigerian livestock species and were in Hardy-Weinberg Equilibrium (HWE). The amino acid changes in these five non-synonymous SNP were all “benign” via Polyphen-2 program. Three SNPs G34C, T699C, and C738G occurred only in Nigerian dogs while C16G, G502A, G503A, and C681A in Nigerian horse. In addition, C50T was detected only in goats and sheep. Conclusion Our study serves as the first to simultaneously investigate the polymorphism of PRNP gene in Nigerian livestock species and provides relevant information that could be adopted in programs targeted at breeding for prion diseases resistance.
