Molecular Genetic Characterization of an Anthrabenzoxocinones Gene Cluster in Streptomyces Sp. FJS31-2 for the Biosynthesis of BE-24566B and Zunyimycin Ale
Yuhong Lü,
Changwu Yue,
Meiyun Shao,
Shengyan Qian,
Ning Liu,
Yuxin Bao,
Miao Wang,
Minghao Liu,
Xiaoqian Li,
Yinyin Wang,
Ying Huang
Affiliations
Yuhong Lü
Guizhou Key Laboratory of Microbial Resources & Drug Development, Zunyi Medical University, Zunyi, Guizhou 563003, China
Changwu Yue
Guizhou Key Laboratory of Microbial Resources & Drug Development, Zunyi Medical University, Zunyi, Guizhou 563003, China
Meiyun Shao
Guizhou Key Laboratory of Microbial Resources & Drug Development, Zunyi Medical University, Zunyi, Guizhou 563003, China
Shengyan Qian
Guizhou Key Laboratory of Microbial Resources & Drug Development, Zunyi Medical University, Zunyi, Guizhou 563003, China
Ning Liu
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
Yuxin Bao
Guizhou Key Laboratory of Microbial Resources & Drug Development, Zunyi Medical University, Zunyi, Guizhou 563003, China
Miao Wang
Guizhou Key Laboratory of Microbial Resources & Drug Development, Zunyi Medical University, Zunyi, Guizhou 563003, China
Minghao Liu
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
Xiaoqian Li
Guizhou Key Laboratory of Microbial Resources & Drug Development, Zunyi Medical University, Zunyi, Guizhou 563003, China
Yinyin Wang
Guizhou Key Laboratory of Microbial Resources & Drug Development, Zunyi Medical University, Zunyi, Guizhou 563003, China
Ying Huang
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
Genome mining is an effective tool used to discover novel natural products from actinomycetes. Genome sequence analysis of Streptomyces sp. FJS31-2 revealed the presence of one putative type II polyketide gene cluster (ABX), which may correspond to type II polyketide products including BE-24566B and its chloro-derivatives. The addition of natural humus acid successfully activated the biosynthsis of the abx gene cluster. BE-24566B and its chloro-derivatives, named zunyimycin A, were also detected. The targeted deletion of the polyketide skeleton synthesis genes such as abxp, abxk, and abxs was performed in the wild strain to identify the gene cluster for BE-24566B biosynthesis.
