Cancer Management and Research (Dec 2020)
Effects of LncRNA HCP5/miR-214-3p/MAPK1 Molecular Network on Renal Cell Carcinoma Cells
Abstract
Jun-Feng Hao,1,* Pei Chen,2,* He-Yi Li,3 Ya-Jing Li,1 Yu-Ling Zhang2 1Department of Nephrology and Blood Purification Center, Jin Qiu Hospital of Liaoning Province (Geriatric Hospital of Liaoning Province), Shenyang City, Liaoning Province 110000, People’s Republic of China; 2Department of Basic Medical Sciences, Jiangsu College of Nursing, Huai’an, Jiangsu Province 223000, People’s Republic of China; 3Department of Ophthalmology, Jin Qiu Hospital of Liaoning Province (Geriatric Hospital of Liaoning Province), Shenyang City, Liaoning Province 110000, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yu-Ling Zhang Tel +86-15162920912Email [email protected]: Recent researches have shown that long non-coding RNA (LncRNA) is often disordered and acts in many carcinomas. Clear cell renal cell carcinoma (ccRCC) is the main reason for carcinoma-related deaths, which are mainly caused by the metastasis. HCP5 is a newly discovered LcnRNA. Early studies have found that HCP5 acts in neoplasm metastasis, but the mechanism of HCP5 in ccRCC is still unclear.Methods: The expression of HCP5 in human renal cell carcinoma (RCC) was detected by real-time quantitative PCR. The biological effect of LncRNAs in proliferation, migration, invasion and metastasis of RCC cells was explored by gain-of-function and loss-of-function tests. The molecular mechanism of LncRNAs was explored by RNA immunoprecipitation and Western blot.Results: qRT-PCR revealed that HCP5 was enhanced in neoplasm tissues of ccRCC patients and correlated with the metastatic characteristics of RCC. Over-expression of HCP5 promoted the proliferation, migration and invasion of renal carcinoma cells. The deletion of HCP5 inhibited the proliferation, migration and invasion of RCC in vitro and the metastasis of RCC in vivo. Mechanically, HCP5 inhibited the growth and metastasis of ccRCC cells by regulating miR-214-3p/MAPK1 axis.Conclusion: HCP5, as a key LncRNA, can promote ccRCC metastasis by regulating miR-214-3p/MAPK1 axis and may be a biomarker and be helpful for judging the prognosis of ccRCC.Keywords: HCP5, miR-214-3p, MAPK1, clear cell renal cell carcinoma
