In Vitro Morphogenesis of Gypsophila: Response to Exogenous Phytohormones

Abstract

The success of in vitro cultivation of plant species depends on factors associated with the induction and control of morphogenesis regarding the regeneration of shoots and roots in the organogenesis process, such as the culture medium composition. Thus, the objective of this work was to investigate whether the concentration of growth regulators in culture medium interferes with the in vitro morphogenesis of gypsophila. ‘Golan’ cultivar was subjected to three culture media (M), which constituted the study treatments: M1) Murashige and Skoog (MS) medium without addition of growth regulators; M2) MS + 1 mg L-1 of benzylaminopurine (BAP) + 0.05 mg L-1 of naphthaleneacetic acid (NAA); M3) MS + 0.05 mg L-1 BAP + 1 mg L-1 NAA. After 45 days, the multiplication rate, plant height and root length were evaluated. The results showed that plantlets produced in M2 medium had a higher multiplication rate. Also, plantlets produced in M1 and M3 media showed higher shoot height and root length. It is concluded that there is a difference in the in vitro morphogenesis of gypsophila according to the concentration of growth regulators in the micropropagation medium.

Keywords

• Gypsophila paniculata L
• Growth regulators
• Micropropagation