Generation of induced pluripotent stem cell lines from three individuals with autism spectrum disorder

Kealan Pugsley; Atefeh Namipashaki; Katerina Vlahos; Kirsten Furley; Alison Graham; Beth P Johnson; Kathryn Kallady; Jia Yi Kuah; Vishnu Priya Mohanakumar Sindhu; Annabelle Suter; Ziarih Hawi; Mark A Bellgrove

Stem Cell Research (Sep 2023)

Generation of induced pluripotent stem cell lines from three individuals with autism spectrum disorder

Kealan Pugsley,
Atefeh Namipashaki,
Katerina Vlahos,
Kirsten Furley,
Alison Graham,
Beth P Johnson,
Kathryn Kallady,
Jia Yi Kuah,
Vishnu Priya Mohanakumar Sindhu,
Annabelle Suter,
Ziarih Hawi,
Mark A Bellgrove

Affiliations

Kealan Pugsley: Turner Institute for Brain and Mental Health and School of Psychological Sciences, Monash University, Melbourne, VIC, Australia
Atefeh Namipashaki: Turner Institute for Brain and Mental Health and School of Psychological Sciences, Monash University, Melbourne, VIC, Australia
Katerina Vlahos: Murdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, VIC, Australia
Kirsten Furley: Department of Paediatrics, Monash Children's Hospital, Melbourne, VIC, Australia
Alison Graham: Murdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, VIC, Australia
Beth P Johnson: Turner Institute for Brain and Mental Health and School of Psychological Sciences, Monash University, Melbourne, VIC, Australia; Department of Paediatrics, Monash Children's Hospital, Melbourne, VIC, Australia
Kathryn Kallady: Turner Institute for Brain and Mental Health and School of Psychological Sciences, Monash University, Melbourne, VIC, Australia
Jia Yi Kuah: Murdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, VIC, Australia
Vishnu Priya Mohanakumar Sindhu: Turner Institute for Brain and Mental Health and School of Psychological Sciences, Monash University, Melbourne, VIC, Australia; Department of Paediatrics, Monash Children's Hospital, Melbourne, VIC, Australia
Annabelle Suter: Murdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, VIC, Australia
Ziarih Hawi: Turner Institute for Brain and Mental Health and School of Psychological Sciences, Monash University, Melbourne, VIC, Australia; Corresponding author.
Mark A Bellgrove: Turner Institute for Brain and Mental Health and School of Psychological Sciences, Monash University, Melbourne, VIC, Australia

Journal volume & issue: Vol. 71
p. 103170

Abstract

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Uncovering the molecular mechanisms of autism spectrum disorder (autism) necessitates development of relevant experimental models that are capable of recapitulating features of the clinical phenotype. Using non-integrative episomal vectors, peripheral blood mononuclear cells derived from three unrelated individuals diagnosed with autism were reprogrammed to induced pluripotent stem cells (iPSCs). The resultant lines exhibited the expected cellular morphology, karyotype, and evidence of pluripotency. These iPSCs constitute a valuable resource to support investigations of the underlying aetiology of autism.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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