Frontiers in Plant Science (Oct 2020)

Genotype-Independent Transformation and Genome Editing of Brassica napus Using a Novel Explant Material

  • Uyen Cao Chu,
  • Sandeep Kumar,
  • Amy Sigmund,
  • Kari Johnson,
  • Yinghong Li,
  • Pamila Vongdeuane,
  • Todd J. Jones

DOI
https://doi.org/10.3389/fpls.2020.579524
Journal volume & issue
Vol. 11

Abstract

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Agrobacterium-mediated transformation of canola (Brassica napus) via hypocotyl segments has been a commonly used method for the past 30 years. While the hypocotyl-based method is well-established, it is not readily adapted to elite germplasm and the prolonged process is not ideal for a production transformation setting. We developed an Agrobacterium-mediated transformation method based on epicotyl and higher stem (internodal) segments that is efficient, rapid and amenable for high-throughput transformation and genome editing. The method has been successfully implemented in multiple canola genotypes. The method appears to be genotype-independent, with varying transformation efficiencies. Internodal segment transformation was used to generate transgenic events as well as CRISPR-Cas9-mediated frameshift gene knockouts.

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