Identification of Renieramycin A as an Antileishmanial Substance in a Marine Sponge Neopetrosia sp.

Abstract

Abstract: The newly developed assay system using recombinant Leishmania amazonensis expressing enhanced green fluorescent protein (La/egfp) has been applied to the screening of Japanese marine sponges for antileishmanial activity. Bioassay-guided fractionation of an active sponge Neopetrosia sp. afforded an active compound which was identified as renieramycin A by spectroscopic analysis. It inhibited La/egfp with an IC50 value of 0.2 μg/mL.

Keywords

• leishmaniasis
• marine sponge
• renieramycin A