Проблемы особо опасных инфекций (Dec 2013)

Construction and Approval of the Test-System for the Detection of Antibodies to Anthrax Agent Using Indirect Fluorescent Immunoassay

  • L. Yu. Aksenova,
  • A. G. Ryazanova,
  • E. V. Zhdanova,
  • O. I. Tsygankova,
  • N. P. Buravtseva,
  • E. I. Eremenko,
  • I. S. Tyumentseva,
  • O. I. Kogotkova,
  • A. A. Zuenko,
  • A. N. Kulichenko

DOI
https://doi.org/10.21055/0370-1069-2013-4-76-78
Journal volume & issue
Vol. 0, no. 4
pp. 76 – 78

Abstract

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Constructed and validated has been the test-system for the detection of antibodies to anthrax agent using indirect fluorescent immunoassay. The panel consists of antigen preparation, positive control sample, negative control, and FITS-labeled rabbit antibodies. Studied have been 10 acapsular B. anthracis strains with different plasmid content and colony morphology. B. anthracis vaccine strain STI-1 is demonstrated to be the optimal one as antigen preparation. Sensitivity of this test-system is not less than 1:40, the working dilution of FITC-conjugate being 1:16. Specificity of the panel has been studied using 100 sera of healthy donors. Approved is 100 % reproducibility of the technique at different time intervals, as well as when carried by different specialists. The test-system allows for confirmation of anthrax diagnosis in humans.

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