Risk Management and Healthcare Policy (Sep 2020)
Molecular Epidemiology and Risk Factors of Blastocystis sp. Infections Among General Populations in Yunnan Province, Southwestern China
Abstract
Yao Deng,1,* Shunxian Zhang,2,* Chaoqun Ning,2 Yongkang Zhou,3 Xuejiao Teng,2 Xiuping Wu,2 Yanhong Chu,2 Yingfang Yu,2 Jiaxu Chen,2 Liguang Tian,2 Wei Wang1 1Key Laboratory of National Health Commission on Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi 214064, Jiangsu Province, People’s Republic of China; 2National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Key Laboratory of National Health Commission on Parasites and Vector Biology, WHO Collaborating Center for Tropical Diseases, Chinese Center for Tropical Diseases Research, Shanghai 200025, People’s Republic of China; 3The First Clinical Medical College of Lanzhou University, Lanzhou 730000, Gansu Province, People’s Republic of China*These authors contributed equally to this workCorrespondence: Liguang TianNational Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Key Laboratory of National Health Commission on Parasites and Vector Biology, WHO Collaborating Center for Tropical Diseases, Chinese Center for Tropical Diseases Research, Shanghai City 200025, People’s Republic of ChinaEmail [email protected] WangKey Laboratory of National Health Commission on Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi City, Jiangsu Province 214064, People’s Republic of ChinaEmail [email protected]: Blastocystis is a common enteric parasite of controversial pathogenic roles in human diseases. Although the prevalence of Blastocystis infections has been investigated in a diverse range of populations, there is little knowledge on the molecular epidemiology and risk factors of Blastocystis infections among general populations in southeastern China.Materials and Methods: A total of 507 individuals were randomly selected in Yunnan province, China from July 2016 to March 2017. Stool specimens were sampled for detection of Blastocystis sp. using PCR assay, and the risk factors of Blastocystis infections were identified. Blastocystis isolates were subtyped, and the associations of Blastocystis infections and subtypes with clinical manifestations were examined.Results: The overall detection rate of Blastocystis sp. was 9.47% (95% CI: 7.13– 12.44%). Toilet type (OR = 3.248, 95% CI: 1.245– 8.473), anemia (OR = 2.601, 95% CI: 1.245– 8.473) and type of daily drinking water (OR = 3.11, 95% CI: 1.557– 6.213) were identified as risk factors of Blastocystis infections; however, Blastocystis infections showed no associations with clinical symptoms. Four subtypes (ST1 to ST4) were characterized in Blastocystis isolates, in which ST3 was predominant (4.73%, 95% CI: 3.2– 6.94%), followed by ST1 (3.16%, 95% CI: 1.95– 5.07%), ST4 (1.38%, 95% CI: 0.07– 2.82%) and ST2 (0.2%, 95% CI: 0– 1.11%). In addition, ST1 subtype infection was found to correlate with anemia (OR = 4.66, 95% CI: 1.631– 14.314).Conclusions: There is a high prevalence of Blastocystis infections among general populations in Yunnan province, southwestern China, and toilet type, anemia and type of daily drinking water are risk factors of Blastocystis infections. ST3 is the dominant subtype of Blastocystis sp. characterized, and ST1 correlates with anemia. Improving hygiene conditions, developing healthy lifestyles and intensifying health education programs are strongly recommended to reduce the prevalence and transmission potential of Blastocystis infections.Keywords: Blastocystis sp., molecular epidemiology, risk factor, subtype, clinical manifestation, southwestern China
