Association of 8-hydroxy-2’-deoxyguanosine with various demographic and biochemical parameter in a group of hemodialysis patients

Abstract

Introduction: Hemodialysis (HD) patients have elevated markers of oxidative stress which is contributed to increased mortality. Objectives: The aim of this study was to determine the association of 8-hydroxy-2’-deoxyguanosine (8- OHdG) as a marker of oxidative stress with various demographic and biochemical parameters in HD patients. Patients and Methods: Eighty-five HD patients (58 men and 27 women; mean ± SD of age was 62.8 ± 13.76 years) who were under HD therapy for at least six months and did not have a systemic infection were enrolled in the study. Serum 8-OHDG was measured by ELISA (enzyme-linked immunosorbent assay) method. Additionally levels of vitamin D, magnesium, and intact parathyroid hormone (PTH) were measured with standard kits. Other clinical and biochemical variables were gathered from patients’ files. Results: The result showed no significant correlation between serum levels of 8-OHDG with measured biochemical and demographic parameters. However, in HD patients with diabetes and hypertension (or combined), a significant reverse correlation between serum albumin and 8-OHdG was detected. Conclusion: In the age group under 40 years, we found a positive and significant correlation between albumin and 8-OHdG. This finding requires further investigation to determine relationship between serum 8-OHdG levels and serum albumin types as reduced and oxidized forms.

Keywords

• end-stage renal disease
• oxidative stress
• 8-hydroxy-2’-deoxyguanosine
• hemodialysis