Metabolic Alterations of the Zebrafish Brain after Acute Alcohol Treatment by 1H Nuclear Magnetic Resonance Spectroscopy

Dong-Cheol Woo; Goo-Young Kim; Hyun-Ju Kim; Eunjung Bang; Hyang-Shuk Rhim; Sang-Young Kim; Do-Wan Lee; Chi-Bong Choi; Youl-Hun Seoung; Bo-Young Choe

doi:10.1155/2013/971914

Journal of Spectroscopy (Jan 2013)

Metabolic Alterations of the Zebrafish Brain after Acute Alcohol Treatment by 1H Nuclear Magnetic Resonance Spectroscopy

Dong-Cheol Woo,
Goo-Young Kim,
Hyun-Ju Kim,
Eunjung Bang,
Hyang-Shuk Rhim,
Sang-Young Kim,
Do-Wan Lee,
Chi-Bong Choi,
Youl-Hun Seoung,
Bo-Young Choe

Affiliations

Dong-Cheol Woo: Department of Radiology, UT Southwestern Medical Center, Dallas, TX, USA
Goo-Young Kim: Department of Molecular Biology, College of Medicine, The Catholic University of Korea, Republic of Korea
Hyun-Ju Kim: Environment & Metabolomics Research Team, Seoul Center, Korea Basic Science Institute, Seoul, Republic of Korea
Eunjung Bang: Environment & Metabolomics Research Team, Seoul Center, Korea Basic Science Institute, Seoul, Republic of Korea
Hyang-Shuk Rhim: Department of Molecular Biology, College of Medicine, The Catholic University of Korea, Republic of Korea
Sang-Young Kim: Department of Biomedical Engineering, Research Institute of Biomedical Engineering, College of Medicine, The Catholic University of Korea, Seoul 137-701, Republic of Korea
Do-Wan Lee: Department of Biomedical Engineering, Research Institute of Biomedical Engineering, College of Medicine, The Catholic University of Korea, Seoul 137-701, Republic of Korea
Chi-Bong Choi: Department of Biomedical Engineering, Research Institute of Biomedical Engineering, College of Medicine, The Catholic University of Korea, Seoul 137-701, Republic of Korea
Youl-Hun Seoung: Department of Biomedical Engineering, Research Institute of Biomedical Engineering, College of Medicine, The Catholic University of Korea, Seoul 137-701, Republic of Korea
Bo-Young Choe: Department of Biomedical Engineering, Research Institute of Biomedical Engineering, College of Medicine, The Catholic University of Korea, Seoul 137-701, Republic of Korea

DOI: https://doi.org/10.1155/2013/971914
Journal volume & issue: Vol. 2013

Abstract

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The purpose of this study is to investigate the metabolic alterations associated with acute alcohol treatment in zebrafish by 1H nuclear magnetic resonance spectroscopy (NMRS). The brain metabolism of zebrafish was investigated after acute alcohol treatment (one-hour long exposure of adult fish to 0.00%, 0.25%, 0.50%, or 1.00% ethyl alcohol) with whole brain extraction. The results of this study showed that glutamate (Glu) was significantly decreased, scyllo-inositol (sIns) showed a small apparent increase only in the highest acute treatment dose group, and myoinositol (mIns) showed a significant decrease. [Glu]/[tCr] and [mIns]/[tCr] levels were significantly reduced regardless of the alcohol dose, and [sIns]/[tCr] was increased in the highest alcohol treatment dose group. The present NMR study revealed that specific metabolites, such as Glu and mIns, were substantially decreased in case of acute alcohol exposed zebrafish brain.

Published in Journal of Spectroscopy

ISSN: 2314-4920 (Print); 2314-4939 (Online)
Publisher: Wiley
Country of publisher: United Kingdom
LCC subjects: Science: Physics: Optics. Light
Website: https://onlinelibrary.wiley.com/journal/9169

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