Single-cell transcriptomics of a dynamic cell behavior in murine airways
Sheldon JJ Kwok,
Daniel T Montoro,
Adam L Haber,
Seok-Hyun Yun,
Vladimir Vinarsky
Affiliations
Sheldon JJ Kwok
Harvard Medical School and Wellman Center for Photomedicine, Massachusetts General Hospital, Cambridge, United States; Harvard-MIT Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, United States
Harvard Medical School and Wellman Center for Photomedicine, Massachusetts General Hospital, Cambridge, United States; Harvard-MIT Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, United States
Center for Regenerative Medicine, Massachusetts General Hospital, Boston, United States; Division of Pulmonary and Critical Care Medicine, Department of Medicine, Massachusetts General Hospital, Boston, United States
Despite advances in high-dimensional cellular analysis, the molecular profiling of dynamic behaviors of cells in their native environment remains a major challenge. We present a method that allows us to couple the physiological behaviors of cells in an intact murine tissue to deep molecular profiling of individual cells. This method enabled us to establish a novel molecular signature for a striking migratory cellular behavior following injury in murine airways.
