The mechanism of Andrena camellia in digesting toxic sugars
Zhen Li,
Shiqing Zhong,
Qiang Huang,
Yong Zhang,
Tianyu Xu,
Wenkai Shi,
Dongsheng Guo,
Zhijiang Zeng
Affiliations
Zhen Li
Honeybee Research Institute, Jiangxi Agricultural University, Nanchang 330045, China; Jiangxi Province Key Laboratory of Honeybee Biology and Beekeeping, Nanchang 330045, China; College of Life Science and Resources and Environment, Yichun University, Yichun 336000, China
Shiqing Zhong
Honeybee Research Institute, Jiangxi Agricultural University, Nanchang 330045, China; Jiangxi Province Key Laboratory of Honeybee Biology and Beekeeping, Nanchang 330045, China
Qiang Huang
Honeybee Research Institute, Jiangxi Agricultural University, Nanchang 330045, China; Jiangxi Province Key Laboratory of Honeybee Biology and Beekeeping, Nanchang 330045, China
Yong Zhang
Honeybee Research Institute, Jiangxi Agricultural University, Nanchang 330045, China; Jiujiang University, Jiujiang 332005, China
Tianyu Xu
Honeybee Research Institute, Jiangxi Agricultural University, Nanchang 330045, China; Jiangxi Province Key Laboratory of Honeybee Biology and Beekeeping, Nanchang 330045, China
Wenkai Shi
Honeybee Research Institute, Jiangxi Agricultural University, Nanchang 330045, China; Jiangxi Province Key Laboratory of Honeybee Biology and Beekeeping, Nanchang 330045, China
Dongsheng Guo
College of Life Science and Resources and Environment, Yichun University, Yichun 336000, China
Zhijiang Zeng
Honeybee Research Institute, Jiangxi Agricultural University, Nanchang 330045, China; Jiangxi Province Key Laboratory of Honeybee Biology and Beekeeping, Nanchang 330045, China; Corresponding author
Summary: Camellia oleifera is an economically and medicinally valuable oilseed crop. Honeybee, the most abundant pollinator, rarely visits C. oleifera because of the toxic sugars in the nectar and pollen. These toxic sugars cannot be fully digested by honeybees and inhibit the process of synthesizing trehalose in honeybees. C. oleifera exhibits self-incompatibility, and its pollination heavily depends on Andrena camellia. However, the mechanism by which A. camellia digests toxic sugars in C. oleifera nectar and pollen remains unknown. Consequently, we identified and validated four single-copy genes (α-N-acetyl galactosamine-like, galactokinase, galactose-1-phosphate uridyltransferase, and UDP-galactose-4′-epimerase, abbreviated as NAGA-like, GALK, GALT, and GALE) essential for detoxifying toxic sugars in vitro. Then, we cloned the four genes into Escherichia coli, and expressed enzyme successfully degraded the toxic sugars. The phylogeny suggests that the genes were conserved and functionally diverged among the evolution. These results provide novel insights into pollinator detoxification during co-evolution.
