Origanum majorana harvested from Al-Soda, Saudi Arabia promotes mitotic arrest and apoptosis in colon cancer cells

Essam H. Ibrahim; Mohammad Y. Alshahrani; Hamed A. Ghramh; Hani Alothaid; Mona Kilany; Kareem Morsy; Attalla F. El-kott; Ramadan Taha; Haitham I. El-Mekkawy; Gamal A. EL-Shaboury; Ahmed A. El-Mansi; Mohammed E.A. Mohammed; Mahmoud A. Sayed; Ibrahim S. Yahia

Journal of King Saud University: Science (Apr 2022)

Origanum majorana harvested from Al-Soda, Saudi Arabia promotes mitotic arrest and apoptosis in colon cancer cells

Essam H. Ibrahim,
Mohammad Y. Alshahrani,
Hamed A. Ghramh,
Hani Alothaid,
Mona Kilany,
Kareem Morsy,
Attalla F. El-kott,
Ramadan Taha,
Haitham I. El-Mekkawy,
Gamal A. EL-Shaboury,
Ahmed A. El-Mansi,
Mohammed E.A. Mohammed,
Mahmoud A. Sayed,
Ibrahim S. Yahia

Affiliations

Essam H. Ibrahim: Biology Department, Faculty of Science, King Khalid University, P.O. Box 9004, Abha 61413, Saudi Arabia; Research Center for Advanced Materials Science (RCAMS), King Khalid University, P.O. Box 9004, Abha 61413, Saudi Arabia; Blood Products Quality Control and Research Department, National Organization for Research and Control of Biologicals, Cairo, Egypt; Corresponding author.
Mohammad Y. Alshahrani: Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Khalid University, P.O. Box 61413, Abha 9088, Saudi Arabia
Hamed A. Ghramh: Biology Department, Faculty of Science, King Khalid University, P.O. Box 9004, Abha 61413, Saudi Arabia; Research Center for Advanced Materials Science (RCAMS), King Khalid University, P.O. Box 9004, Abha 61413, Saudi Arabia; Unit of Bee Research and Honey Production, Faculty of Science, King Khalid University, P.O. Box 9004, Abha 61413, Saudi Arabia
Hani Alothaid: Department of Basic Sciences, Faculty of Applied Medical Sciences, Al-Baha University, Al-Baha, Saudi Arabia
Mona Kilany: Research Center for Advanced Materials Science (RCAMS), King Khalid University, P.O. Box 9004, Abha 61413, Saudi Arabia; Department of Microbiology, National Organization for Drug Control and Research (NODCAR), Cairo, Egypt
Kareem Morsy: Biology Department, Faculty of Science, King Khalid University, P.O. Box 9004, Abha 61413, Saudi Arabia; Department of Zoology, Faculty of Science, Cairo University, 12613 Cairo, Egypt
Attalla F. El-kott: Biology Department, Faculty of Science, King Khalid University, P.O. Box 9004, Abha 61413, Saudi Arabia; Department of Zoology, Faculty of Science, Damanhour University, Damanhour, Egypt
Ramadan Taha: Biology Department, Faculty of Science, King Khalid University, P.O. Box 9004, Abha 61413, Saudi Arabia; Department of Clinical Pathology, Faculty of Veterinary Medicine, Suez Canal University, Egypt
Haitham I. El-Mekkawy: Biology Department, Faculty of Science, King Khalid University, P.O. Box 9004, Abha 61413, Saudi Arabia
Gamal A. EL-Shaboury: Biology Department, Faculty of Science, King Khalid University, P.O. Box 9004, Abha 61413, Saudi Arabia
Ahmed A. El-Mansi: Biology Department, Faculty of Science, King Khalid University, P.O. Box 9004, Abha 61413, Saudi Arabia; Zoology Dept., Faculty of Science, Mansoura University, Mansoura, Egypt
Mohammed E.A. Mohammed: Research Center for Advanced Materials Science (RCAMS), King Khalid University, P.O. Box 9004, Abha 61413, Saudi Arabia; Unit of Bee Research and Honey Production, Faculty of Science, King Khalid University, P.O. Box 9004, Abha 61413, Saudi Arabia; School of Allied Health Science, Faculty of Health and Life Sciences, De Montfort University, The Gateway, Leicester LE1 9BH, United Kingdom
Mahmoud A. Sayed: Research Center for Advanced Materials Science (RCAMS), King Khalid University, P.O. Box 9004, Abha 61413, Saudi Arabia; Physics Department, Faculty of Science, King Khalid University, P. O. Box 9004, Abha 61413, Abha, Saudi Arabia; Physics Department, Faculty of Science, Al-Azhar University, P. O. 71452, Assiut, Egypt
Ibrahim S. Yahia: Research Center for Advanced Materials Science (RCAMS), King Khalid University, P.O. Box 9004, Abha 61413, Saudi Arabia; Physics Department, Faculty of Science, King Khalid University, P. O. Box 9004, Abha 61413, Abha, Saudi Arabia; Nanoscience Laboratory for Environmental and Bio-medical Applications (NLEBA), Semiconductor Lab., Metallurgical Lab.1, Department of Physics, Faculty of Education, Ain Shams University, Roxy, 11757 Cairo, Egypt

Journal volume & issue: Vol. 34, no. 3
p. 101878

Abstract

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Colorectal cancer is reported as the third major incentive of cancer doom. The present work is meant to examine the anticancer potential of Origanum majorana leaf acetone extract (OMAE) to fight HT-29 human colon cancer cells. Biomolecules in OMAE were examined using Fourier Transform Infrared (FT-IR) spectroscopy. Reactive oxygen species (ROS) in OMAE was determined using an immunosobant assay. The cytotoxic effect of OMAE was tested by MTT assay. p53 gene expression level of OMAE-tread cells was measured using quantitative real-time PCR (qPCR). Apoptotic and cell cycle arrest effects of OMAE on HT-29 were analyzed by flow cytometry. Results revealed the presence of many functional groups and considerable amount of ROS in the extract. The extract could raise p53 expression level five folds over control. OMAE arrested the HT-29 at G2/M phase. OMAE has an apoptotic effect rather than necrotic effects Our discoveries give solid proof that O. majorana acetone extract has a capacity to stop colon cancer activity, at least, through the enlistment of cell division arrest as well as apoptosis. These findings can suggest the use of OMAE as a natural therapeutic candidate against the colon cancer.

Published in Journal of King Saud University: Science

ISSN: 1018-3647 (Print)
Publisher: Elsevier
Country of publisher: Saudi Arabia
LCC subjects: Science: Science (General)
Website: http://www.journals.elsevier.com/journal-of-king-saud-university-science/

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