Evaluation of Transforming Growth Factor Beta 1 and Curcumin on Proliferation and Differentiation of Nasal-Derived Chondrocyte Seeded on the Fibrin Glue Scaffold

Abstract

Introduction: Natural biomaterials and growth factors are key factors in tissue engineering. The objective of the present study was to evaluate transforming growth factor beta 1 (TGF-β1) and curcumin on proliferation and differentiation of nasal-derived chondrocyte seeded on the fibrin glue scaffold. Methods: Chondrocytes were isolated from nasal samples. Nasal-derived chondrocytes were seeded on fibrin glue at chondrogenic induction medium for 2 weeks. In this study, the effects of various concentrations of curcumin and TGF-β1 on the survival and proliferation of chondrocytes seeded on fibrin biomaterial were assessed by MTT assays. Also, chondrocytespecific gene expression was assessed by real-time polymerase chain reaction (PCR). Results: There were significant differences among the group treated with curcumin 10 μg compared to other groups with regard to cell viability. Also, gene expression of collagen type II, aggrecan, and SOX9 in the chondrocytes seeded on fibrin biomaterial containing the growth factor TGF-β1 significantly differed from those of curcumin and control group. Conclusion: Our results indicate that TGF-β1 and natural biomaterial of curcumin can be used effectively in chondrogenic viability and differentiation of nasal-derived chondrocyte.

Keywords

• transforming growth factor beta 1
• curcumin
• chondrocytes
• 3-(4
• 5-dimethylthiazol-2-yl)-2
• 5-diphenyl tetrazolium bromide
• real-time pcr