Nature Communications (Oct 2016)
Monitoring peripheral nerve degeneration in ALS by label-free stimulated Raman scattering imaging
- Feng Tian,
- Wenlong Yang,
- Daniel A. Mordes,
- Jin-Yuan Wang,
- Johnny S. Salameh,
- Joanie Mok,
- Jeannie Chew,
- Aarti Sharma,
- Ester Leno-Duran,
- Satomi Suzuki-Uematsu,
- Naoki Suzuki,
- Steve S. Han,
- Fa-Ke Lu,
- Minbiao Ji,
- Rosanna Zhang,
- Yue Liu,
- Jack Strominger,
- Neil A. Shneider,
- Leonard Petrucelli,
- X. Sunney Xie,
- Kevin Eggan
Affiliations
- Feng Tian
- Department of Stem Cell and Regenerative Biology, Harvard Stem Cell Institute, Harvard University
- Wenlong Yang
- Department of Chemistry and Chemical Biology, Harvard University
- Daniel A. Mordes
- Department of Stem Cell and Regenerative Biology, Harvard Stem Cell Institute, Harvard University
- Jin-Yuan Wang
- Department of Stem Cell and Regenerative Biology, Harvard Stem Cell Institute, Harvard University
- Johnny S. Salameh
- Department of Neurology, University of Massachusetts Memorial Medical Center
- Joanie Mok
- Department of Stem Cell and Regenerative Biology, Harvard Stem Cell Institute, Harvard University
- Jeannie Chew
- Department of Neuroscience, Mayo Clinic
- Aarti Sharma
- Department of Neurology, Columbia University Medical Center
- Ester Leno-Duran
- Department of Stem Cell and Regenerative Biology, Harvard Stem Cell Institute, Harvard University
- Satomi Suzuki-Uematsu
- Department of Stem Cell and Regenerative Biology, Harvard Stem Cell Institute, Harvard University
- Naoki Suzuki
- Department of Stem Cell and Regenerative Biology, Harvard Stem Cell Institute, Harvard University
- Steve S. Han
- Department of Stem Cell and Regenerative Biology, Harvard Stem Cell Institute, Harvard University
- Fa-Ke Lu
- Department of Chemistry and Chemical Biology, Harvard University
- Minbiao Ji
- Department of Chemistry and Chemical Biology, Harvard University
- Rosanna Zhang
- Department of Stem Cell and Regenerative Biology, Harvard Stem Cell Institute, Harvard University
- Yue Liu
- Department of Stem Cell and Regenerative Biology, Harvard Stem Cell Institute, Harvard University
- Jack Strominger
- Department of Stem Cell and Regenerative Biology, Harvard Stem Cell Institute, Harvard University
- Neil A. Shneider
- Department of Neurology, Columbia University Medical Center
- Leonard Petrucelli
- Department of Neuroscience, Mayo Clinic
- X. Sunney Xie
- Department of Chemistry and Chemical Biology, Harvard University
- Kevin Eggan
- Department of Stem Cell and Regenerative Biology, Harvard Stem Cell Institute, Harvard University
- DOI
- https://doi.org/10.1038/ncomms13283
- Journal volume & issue
-
Vol. 7,
no. 1
pp. 1 – 15
Abstract
Sensitive and label-free imaging methods to visualize nerve degeneration are currently lacking. Here authors show that stimulated Raman scattering (SRS) microscopy can be used to monitor peripheral nerve degeneration in mouse models of amyotrophic lateral sclerosis (ALS) and in postmortem tissue from ALS patients.
