Important mitochondrial proteins in human omental adipose tissue show reduced expression in obesity
Peter W. Lindinger,
Martine Christe,
Alex N. Eberle,
Beatrice Kern,
Ralph Peterli,
Thomas Peters,
Kamburapola J.I. Jayawardene,
Ian M. Fearnley,
John E. Walker
Affiliations
Peter W. Lindinger
Laboratory of Endocrinology, Department of Biomedicine, University Hospital and University Children’s Hospital, University of Basel, Basel CH-4031, Switzerland
Martine Christe
Laboratory of Endocrinology, Department of Biomedicine, University Hospital and University Children’s Hospital, University of Basel, Basel CH-4031, Switzerland
Alex N. Eberle
Laboratory of Endocrinology, Department of Biomedicine, University Hospital and University Children’s Hospital, University of Basel, Basel CH-4031, Switzerland
Beatrice Kern
Department of Surgery, St. Claraspital, Basel CH-4059, Switzerland
Ralph Peterli
Department of Surgery, St. Claraspital, Basel CH-4059, Switzerland
Thomas Peters
Interdisciplinary Center of Nutritional and Metabolic Diseases, St. Claraspital, Kleinriehenstrasse 30, Basel CH-4058, Switzerland
Kamburapola J.I. Jayawardene
MRC Mitochondrial Biology Unit, Welcome Trust/MRC Building, Hills Road, Cambridge CB2 0XY, UK
Ian M. Fearnley
MRC Mitochondrial Biology Unit, Welcome Trust/MRC Building, Hills Road, Cambridge CB2 0XY, UK
John E. Walker
MRC Mitochondrial Biology Unit, Welcome Trust/MRC Building, Hills Road, Cambridge CB2 0XY, UK
Obesity is associated with impaired mitochondrial function. This study compares mitochondrial protein expression in omental fat in obese and non-obese humans. Omental adipose tissue was obtained by surgical biopsy, adipocytes were purified and mitochondria isolated. Using anion-exchange chromatography, SDS-PAGE and mass-spectrometry, 128 proteins with potentially different abundances in patient groups were identified, 62 of the 128 proteins are mainly localized in the mitochondria. Further quantification of 12 of these 62 proteins by immune dot blot analysis revealed four proteins citrate synthase, HADHA, LETM1 and mitofilin being inversely associated with BMI, and mitofilin being inversely correlated with gender.
