Fast Assessment of Quality of Water Containing Inorganic Pollutants Using Laser Biospeckles in Microbioassay

Abstract

Recently, bioassay techniques have been gaining prominence in assessing water toxicity, offering comprehensive evaluations without identifying the individual chemical component. However, microscopic observation is a crucial component in microbioassays to know the critical features of the targeted microorganisms. However, as the microorganism’s size becomes smaller, observation becomes more difficult due to the narrower focal depth of the imaging system. To address this challenge, we propose a novel laser biospeckle non-imaging technique utilizing biospeckle patterns generated by microorganisms, enabling non-imaging assessments of their swimming ability. Paramecium and Euglena were used as microorganisms. Paramecium and Euglena were subjected to varying concentrations of heavy metal pollutants (Zn(NO3)2·6H2O and FeSO4·7H2O), and their swimming activity was quantified using a dynamic biospeckle analysis. The results show a concentration-dependent effect of Zn on both species, leading to decreased swimming ability at increased concentration. Conversely, Fe exhibited varying effects on Paramecia and Euglena, with the latter displaying tolerance at lower concentrations but a notable response at higher concentrations. The advantage of the method is that owing to the non-imaging system, an enormous number of microorganisms can be processed. Moreover, the method allows for an immediate and statistically significant estimation of their swimming ability in response to environmental pollution.

Keywords

• microbioassay
• biospeckle
• microorganisms
• Euglena
• Paramecia
• water toxicity