Protective role of Dodonaea viscosa extract against streptozotocin-induced hepatotoxicity and nephrotoxicity in rats

Ahmed Z. Alanazi; Salim S. Al-Rejaie; Mohammed M. Ahmed; Khalid Alhazzani; Khaled Alhosaini; Homood M. As Sobeai; Sary Alsanea; Perwez Alam; Omer M. Almarfadi; Ali S. Alqahtani; Abdullah S. Alhamed; Mohammed Alqinyah; Hussain N. Alhamami; Mohammed F. Almutery; Mohamed Mohany

Saudi Pharmaceutical Journal (Aug 2023)

Protective role of Dodonaea viscosa extract against streptozotocin-induced hepatotoxicity and nephrotoxicity in rats

Ahmed Z. Alanazi,
Salim S. Al-Rejaie,
Mohammed M. Ahmed,
Khalid Alhazzani,
Khaled Alhosaini,
Homood M. As Sobeai,
Sary Alsanea,
Perwez Alam,
Omer M. Almarfadi,
Ali S. Alqahtani,
Abdullah S. Alhamed,
Mohammed Alqinyah,
Hussain N. Alhamami,
Mohammed F. Almutery,
Mohamed Mohany

Affiliations

Ahmed Z. Alanazi: Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, P.O. Box 55760, Riyadh 1145, Saudi Arabia; Corresponding author at: Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Building 23 (College of Dentistry), 1st floor, Office 1A 31, P.O. Box 2457, Riyadh 11451, Saudi Arabia.
Salim S. Al-Rejaie: Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, P.O. Box 55760, Riyadh 1145, Saudi Arabia
Mohammed M. Ahmed: Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, P.O. Box 55760, Riyadh 1145, Saudi Arabia
Khalid Alhazzani: Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, P.O. Box 55760, Riyadh 1145, Saudi Arabia
Khaled Alhosaini: Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, P.O. Box 55760, Riyadh 1145, Saudi Arabia
Homood M. As Sobeai: Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, P.O. Box 55760, Riyadh 1145, Saudi Arabia
Sary Alsanea: Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, P.O. Box 55760, Riyadh 1145, Saudi Arabia
Perwez Alam: Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 55760, Riyadh 1145, Saudi Arabia
Omer M. Almarfadi: Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 55760, Riyadh 1145, Saudi Arabia
Ali S. Alqahtani: Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 55760, Riyadh 1145, Saudi Arabia
Abdullah S. Alhamed: Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, P.O. Box 55760, Riyadh 1145, Saudi Arabia
Mohammed Alqinyah: Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, P.O. Box 55760, Riyadh 1145, Saudi Arabia
Hussain N. Alhamami: Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, P.O. Box 55760, Riyadh 1145, Saudi Arabia
Mohammed F. Almutery: Department of Pathology and Laboratory Medicine, College of Medicine, King Saud, University Medical City, King Saud University, Riyadh, Saudi Arabia
Mohamed Mohany: Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, P.O. Box 55760, Riyadh 1145, Saudi Arabia

Journal volume & issue: Vol. 31, no. 8
p. 101669

Abstract

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Previous investigations have shown that D. viscosa herbal extract is often used to treat a variety of diseases. Therefore, the purpose of this study was to investigate any additional potential impacts on rat liver and kidney damage induced by diabetes. Streptozotocin (STZ) (60 mg/kg/day) was given as a single dosage to cause type 1 diabetes. After then, diabetic rats received oral doses of D. viscosa for four weeks at 150 and 300 mg/kg/day. Blood, liver, and kidney tissues were collected at the end of the treatment and examined. Analysis was made of the serum lipid profile, liver, and kidney functions, as well as blood biochemistry. Moreover, the levels of tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), interleukin-1 beta (IL-1β), prostaglandin E-2 (PGE-2), and nitric oxide (NO) were estimated in serum. In liver and kidney samples, thiobarbituric acid reactive substances (TBARs) and reduced glutathione (GSH), as well as the pro-inflammatory cytokines and enzymatic activities of glutathione peroxidase (GPx), glutathione reeducates (GR), glutathione-S-transferase (GST), catalase (CAT), and superoxide dismutase (SOD) were analyzed. Histological changes in liver and kidney cross-sections were also observed. Our findings demonstrated that D. viscosa dramatically decreased pro-inflammatory indicators in blood, kidney, and liver tissues as well as blood glucose, and restored insulin levels, and lipid profiles. Additionally, it significantly raises the antioxidant enzyme activity SOD, CAT, GPx, and GST, while significantly lowering TBARs levels. The above-mentioned biochemical changes that took place in tissues were further supported by histological alterations. These findings imply that D. viscosa protects against STZ-induced hyperglycemia, aberrant lipid synthesis, and oxidative stress and that these benefits may be mediated by interacting with various targets to increase the levels of antioxidant enzymes in the liver and kidneys. Its mode of action and safety for use as medicine against various metabolic problems caused by diabetes require more research.

Published in Saudi Pharmaceutical Journal

ISSN: 1319-0164 (Print)
Publisher: Elsevier
Country of publisher: Saudi Arabia
LCC subjects: Medicine: Therapeutics. Pharmacology
Website: http://www.journals.elsevier.com/saudi-pharmaceutical-journal/

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