Frontiers in Photonics (Feb 2024)
Automatic identification and analysis of cells using digital holographic microscopy and Sobel segmentation
- Zihan Xiong,
- Zihan Xiong,
- Zihan Xiong,
- Zihan Xiong,
- Lan Yu,
- Lan Yu,
- Lan Yu,
- Lan Yu,
- Sha An,
- Sha An,
- Sha An,
- Sha An,
- Juanjuan Zheng,
- Juanjuan Zheng,
- Juanjuan Zheng,
- Juanjuan Zheng,
- Ying Ma,
- Ying Ma,
- Ying Ma,
- Ying Ma,
- Vicente Micó,
- Peng Gao,
- Peng Gao,
- Peng Gao,
- Peng Gao
Affiliations
- Zihan Xiong
- Hangzhou Institute of Technology, Xidian University, Hangzhou, China
- Zihan Xiong
- School of Physics, Xidian University, Xi’an, China
- Zihan Xiong
- Key Laboratory of Optoelectronic Perception of Complex Environment, Ministry of Educationy, Xi’an, China
- Zihan Xiong
- Engineering Research Center of Functional Nanomaterials, Universities of Shaanxi Province, Xi’an, China
- Lan Yu
- Hangzhou Institute of Technology, Xidian University, Hangzhou, China
- Lan Yu
- School of Physics, Xidian University, Xi’an, China
- Lan Yu
- Key Laboratory of Optoelectronic Perception of Complex Environment, Ministry of Educationy, Xi’an, China
- Lan Yu
- Engineering Research Center of Functional Nanomaterials, Universities of Shaanxi Province, Xi’an, China
- Sha An
- Hangzhou Institute of Technology, Xidian University, Hangzhou, China
- Sha An
- School of Physics, Xidian University, Xi’an, China
- Sha An
- Key Laboratory of Optoelectronic Perception of Complex Environment, Ministry of Educationy, Xi’an, China
- Sha An
- Engineering Research Center of Functional Nanomaterials, Universities of Shaanxi Province, Xi’an, China
- Juanjuan Zheng
- Hangzhou Institute of Technology, Xidian University, Hangzhou, China
- Juanjuan Zheng
- School of Physics, Xidian University, Xi’an, China
- Juanjuan Zheng
- Key Laboratory of Optoelectronic Perception of Complex Environment, Ministry of Educationy, Xi’an, China
- Juanjuan Zheng
- Engineering Research Center of Functional Nanomaterials, Universities of Shaanxi Province, Xi’an, China
- Ying Ma
- Hangzhou Institute of Technology, Xidian University, Hangzhou, China
- Ying Ma
- School of Physics, Xidian University, Xi’an, China
- Ying Ma
- Key Laboratory of Optoelectronic Perception of Complex Environment, Ministry of Educationy, Xi’an, China
- Ying Ma
- Engineering Research Center of Functional Nanomaterials, Universities of Shaanxi Province, Xi’an, China
- Vicente Micó
- Departamento de Óptica y de Optometría y Ciencias de la Visión, Universidad de Valencia, Burjassot, Spain
- Peng Gao
- Hangzhou Institute of Technology, Xidian University, Hangzhou, China
- Peng Gao
- School of Physics, Xidian University, Xi’an, China
- Peng Gao
- Key Laboratory of Optoelectronic Perception of Complex Environment, Ministry of Educationy, Xi’an, China
- Peng Gao
- Engineering Research Center of Functional Nanomaterials, Universities of Shaanxi Province, Xi’an, China
- DOI
- https://doi.org/10.3389/fphot.2024.1359595
- Journal volume & issue
-
Vol. 5
Abstract
Counting and analyzing of blood cells, as well as their subcellular structures, are indispensable for understanding biological processes, studying cell functions, and diagnosing diseases. In this paper, we combine digital holographic microscopy with cell segmentation guided by the Sobel operator using Dice coefficients for automatic threshold selection and aimed to automatic counting and analysis of blood cells in flow and different kinds of cells in the static state. We demonstrate the proposed method with automatic counting and analyzing rat red blood cells (RBCS) flowing in a microfluidic device, extracting quickly and accurately the size, concentration, and dry mass of the sample in a label-free manner. The proposed technique was also demonstrated for automatic segmentation of different cell types, such as COS7 and Siha. This method can help us in blood inspection, providing pathological information in disease diagnosis and treatment.
Keywords
- quantitative phase microscopy (QPM)
- digital holographic microscopy (DHM)
- cell segmentation
- cell characteristic parameters
- Sobel operator
