Simultaneous Determination of Four Marker Compounds in <i>Lobelia chinensis</i> Lour. Extract by HPLC-PDA

Abstract

Lobelia chinensis Lour. (L. chinensis) has traditionally been used as a treatment for snake bites, high fever, jaundice, edema, and diarrhea, and modern studies have reported its anti-inflammatory, antioxidant, and antiviral activities. L. chinensis contains various compounds, such as flavonoids and coumarins, and its flavonoid components have been identified in many studies. In this study, a high-performance liquid chromatograph equipped with a photodiode array (PDA) detector and an Aegispak C18-L reverse-phase column (4.6 mm × 250 mm i.d., 5 μm) was used to simultaneously analyze four marker components in L. chinensis for standardization purposes. HPLC-PDA (detection at 340 nm), performed using a 0.1% formic acid-water/0.1% formic acid-acetonitrile gradient, separated the four marker compounds: luteolin-7-O-β-d-glucuronopyranosyl (1→2)-O-β-d-glucuronopyranoside, clerodendrin, chrysoeriol-7-O-diglucuronide, and diosmin. The developed analytical method showed excellent linearity values (r2 > 0.9991), limits of detection (LODs: 0.376–2.152 μg/mL), limits of quantification (LOQs: 1.147–6.521 μg/mL), intra- and inter-day precisions (RSD L. chinensis.

Keywords

• <i>Lobelia chinensis</i> Lour.
• d-glucuronopyranosyl (1→2)-O-β-d-glucuronopyranoside%22%2C%22default_operator%22%3A%22AND%22%7D%7D%7D"> luteolin-7-<i>O</i>-β-<span style="font-variant: small-caps">d</span>-glucuronopyranosyl (1→2)-<i>O</i>-β-<span style="font-variant: small-caps">d</span>-glucuronopyranoside
• clerodendrin
• chrysoeriol-7-<i>O</i>-diglucuronide
• diosmin
• HPLC-PDA