PEG-Functionalized Magnetite Nanoparticles for Modulation of Microbial Biofilms on Voice Prosthesis
Mara Caciandone,
Adelina-Gabriela Niculescu,
Aurelian Radu Roșu,
Valentina Grumezescu,
Irina Negut,
Alina Maria Holban,
Ovidiu Oprea,
Bogdan Ștefan Vasile,
Alexandra Cătălina Bîrcă,
Alexandru Mihai Grumezescu,
Miruna Silvia Stan,
Alina Georgiana Anghel,
Ion Anghel
Affiliations
Mara Caciandone
“Carol Davila” University of Medicine and Pharmacy, 050474 Bucharest, Romania
Adelina-Gabriela Niculescu
Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, Politehnica University of Bucharest, 011061 Bucharest, Romania
Aurelian Radu Roșu
Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, Politehnica University of Bucharest, 011061 Bucharest, Romania
Valentina Grumezescu
Lasers Department, National Institute for Lasers, Plasma and Radiation Physics, 077125 Magurele, Romania
Irina Negut
Lasers Department, National Institute for Lasers, Plasma and Radiation Physics, 077125 Magurele, Romania
Alina Maria Holban
Department of Microbiology and Immunology, Faculty of Biology, University of Bucharest, 077206 Bucharest, Romania
Ovidiu Oprea
Department of Inorganic Chemistry, Physical Chemistry and Electrochemistry, Faculty of Applied Chemistry and Materials Science, Politehnica University of Bucharest, 011061 Bucharest, Romania
Bogdan Ștefan Vasile
Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, Politehnica University of Bucharest, 011061 Bucharest, Romania
Alexandra Cătălina Bîrcă
Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, Politehnica University of Bucharest, 011061 Bucharest, Romania
Alexandru Mihai Grumezescu
Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, Politehnica University of Bucharest, 011061 Bucharest, Romania
Miruna Silvia Stan
Academy of Romanian Scientists, Ilfov No. 3, 050044 Bucharest, Romania
Alina Georgiana Anghel
“Carol Davila” University of Medicine and Pharmacy, 050474 Bucharest, Romania
Ion Anghel
“Carol Davila” University of Medicine and Pharmacy, 050474 Bucharest, Romania
This study reports the fabrication of nanostructured coatings based on magnetite, polyethyleneglycol, and biologically active molecule (polymyxin B-PM) for producing biofilm-resistant surfaces (voice prosthesis). Magnetite nanoparticles (MNPs) have been synthesized and functionalized using a co-precipitation method and were further deposited into thin coatings using the matrix-assisted pulsed laser evaporation (MAPLE) technique. The obtained nanoparticles and coatings were characterized by X-ray diffraction (XRD), thermogravimetric analysis with differential scanning calorimetry (TGA-DSC), scanning electron microscopy (SEM), transmission electron microscopy with selected area electron diffraction (TEM-SAED), Fourier-transform infrared spectroscopy (FT-IR), and infrared microscopy (IRM). Their antibiofilm activity was tested against relevant Staphylococcus aureus and Pseudomonas aeruginosa bacterial strains. The Fe3O4@PEG/PM surface of modified voice prosthesis sections reduced the number of CFU/mL up to four orders of magnitude in the case of S. aureus biofilm. A more significant inhibitory effect is noticed in the case of P. aeruginosa up to five folds. These results highlight the importance of new Fe3O4@PEG/PM in the biomedical field.
