Virology Journal (Sep 2006)

Circulating viral core and E1 antigen levels as supplemental markers for HCV Chronic hepatitis

  • El Awady Mostafa K,
  • El Abd Yasmine S,
  • Shoeb Hussein A,
  • Tabll Ashraf A,
  • Hosny Alaa El Din MS,
  • El Shenawy Reem M,
  • Atef Khaled,
  • Bader El Din Noha G,
  • Bahgat Mahmoud M

DOI
https://doi.org/10.1186/1743-422X-3-67
Journal volume & issue
Vol. 3, no. 1
p. 67

Abstract

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Abstract The performance of polyclonal monospecific rabbit anti-sera raised against synthetic peptides derived from conserved HCV sequences of genotype 4 was evaluated for efficient detection of viral core and E1 antigens in circulating immune complexes (ICs) precipitated from 65 serum samples of HCV patients. The infection was established in those patients by the presence of HCV RNA in their sera. A novel enzyme-linked immunosorbent assay (ELISA) was developed for the detection of HCV core and E1 antigen in serum samples. Western blot analyses were used to demonstrate the presence of the core and E1 target antigen in serum samples. The mean OD readings of both core and E1 antigens were significantly higher (P