Dynamics of Infections in Cattle and <i>Rhipicephalus microplus</i>: A Preliminary Study
Elianne Piloto-Sardiñas,
Angélique Foucault-Simonin,
Alejandra Wu-Chuang,
Lourdes Mateos-Hernández,
Roxana Marrero-Perera,
Lianet Abuin-Denis,
Lisset Roblejo-Arias,
Cristian Díaz-Corona,
Zbigniew Zając,
Joanna Kulisz,
Aneta Woźniak,
Sara Moutailler,
Belkis Corona-González,
Alejandro Cabezas-Cruz
Affiliations
Elianne Piloto-Sardiñas
ANSES, INRAE, Ecole Nationale Vétérinaire d’Alfort, UMR BIPAR, Laboratoire de Santé Animale, Maisons-Alfort 94700, France
Angélique Foucault-Simonin
ANSES, INRAE, Ecole Nationale Vétérinaire d’Alfort, UMR BIPAR, Laboratoire de Santé Animale, Maisons-Alfort 94700, France
Alejandra Wu-Chuang
ANSES, INRAE, Ecole Nationale Vétérinaire d’Alfort, UMR BIPAR, Laboratoire de Santé Animale, Maisons-Alfort 94700, France
Lourdes Mateos-Hernández
ANSES, INRAE, Ecole Nationale Vétérinaire d’Alfort, UMR BIPAR, Laboratoire de Santé Animale, Maisons-Alfort 94700, France
Roxana Marrero-Perera
Direction of Animal Health, National Center for Animal and Plant Health, Carretera de Tapaste y Autopista Nacional, Apartado Postal 10, San José de las Lajas 32700, Mayabeque, Cuba
Lianet Abuin-Denis
ANSES, INRAE, Ecole Nationale Vétérinaire d’Alfort, UMR BIPAR, Laboratoire de Santé Animale, Maisons-Alfort 94700, France
Lisset Roblejo-Arias
Direction of Animal Health, National Center for Animal and Plant Health, Carretera de Tapaste y Autopista Nacional, Apartado Postal 10, San José de las Lajas 32700, Mayabeque, Cuba
Cristian Díaz-Corona
Direction of Animal Health, National Center for Animal and Plant Health, Carretera de Tapaste y Autopista Nacional, Apartado Postal 10, San José de las Lajas 32700, Mayabeque, Cuba
Zbigniew Zając
Department of Biology and Parasitology, Medical University of Lublin, Radziwiłłowska 11 St., 20-080 Lublin, Poland
Joanna Kulisz
Department of Biology and Parasitology, Medical University of Lublin, Radziwiłłowska 11 St., 20-080 Lublin, Poland
Aneta Woźniak
Department of Biology and Parasitology, Medical University of Lublin, Radziwiłłowska 11 St., 20-080 Lublin, Poland
Sara Moutailler
ANSES, INRAE, Ecole Nationale Vétérinaire d’Alfort, UMR BIPAR, Laboratoire de Santé Animale, Maisons-Alfort 94700, France
Belkis Corona-González
Direction of Animal Health, National Center for Animal and Plant Health, Carretera de Tapaste y Autopista Nacional, Apartado Postal 10, San José de las Lajas 32700, Mayabeque, Cuba
Alejandro Cabezas-Cruz
ANSES, INRAE, Ecole Nationale Vétérinaire d’Alfort, UMR BIPAR, Laboratoire de Santé Animale, Maisons-Alfort 94700, France
Tick-borne pathogens (TBPs) pose a significant threat to livestock, including bovine species. This study aimed to investigate TBPs in cattle and ticks across four sampling points, utilizing real-time microfluidic PCR. The results revealed that Rhipicephalus microplus ticks were found infesting all animals. Among the detected TBPs in cattle, Anaplasma marginale was the most frequently identified, often as a single infection, although mixed infections involving Rickettsia felis, uncharacterized Rickettsia sp., and Anaplasma sp. were also observed. In ticks, A. marginale was predominant, along with R. felis, Rickettsia sp., and Ehrlichia sp. It is noteworthy that although A. marginale consistently infected all cattle during various sampling times, this pathogen was not detected in all ticks. This suggests a complex dynamic of pathogen acquisition by ticks. A phylogenetic analysis focused on the identification of Anaplasma species using amplified 16S rDNA gene fragments revealed the presence of A. marginale and Anaplasma platys strains in bovines. These findings underscore the presence of multiple TBPs in both cattle and ticks, with A. marginale being the most prevalent. Understanding the dynamics and phylogenetics of TBPs is crucial for developing effective control strategies to mitigate tick-borne diseases in livestock.
