Journal of Inflammation Research (Oct 2021)
Curcumae Ameliorates Diethylnitrosamine-Induced Hepatocellular Carcinoma via Alteration of Oxidative Stress, Inflammation and Gut Microbiota
Abstract
Yunyan Zhang,1 Xuelian Li,2 Xinghua Li3 1Department of Laboratory Medicine, The First People’s Hospital of Lianyungang City, Lianyungang City, Jiangsu, 222002, People’s Republic of China; 2Department of Laboratory Medicine, The Fourth People’s Hospital of Lianyungang City, Lianyungang City, Jiangsu, 222002, People’s Republic of China; 3Department of Blood Transfusion, The First People’s Hospital of Lianyungang City, Lianyungang City, Jiangsu, 222002, People’s Republic of ChinaCorrespondence: Xinghua LiDepartment of Blood Transfusion, The First People’s Hospital of Lianyungang City, Lianyungang City, Jiangsu, 222002, People’s Republic of ChinaEmail [email protected]: Nonalcoholic fatty liver disease (NAFLD) increased the risk factor of hepatocellular carcinoma (HCC). NAFLD induces the hepatic-related cancer deaths mostly in middle-aged men. NAFLD enhanced the inflammatory reaction and oxidative stress in the hepatic tissue. Curcumae exhibited the anti-inflammatory and antioxidant effects. In this study, we made an attempt to scrutinize the protective effect of curcumae on obesity-induced HCC via alteration of inflammation, oxidative stress and gut microbiota.Methods: The rats used in this experiment were Wistar rats, 100 mg/kg intraperitoneal injection of diethylnitrosamine (hepatic carcinogen) was used at 2 weeks. After 6 weeks of the experimental study, the rats were randomly divided into high-fat diet (HFD) with or without curcumae-treated group rats and received the treatment for 22 weeks. Hepatic, non-hepatic, cardiac, antioxidant, pro-inflammatory and inflammatory were estimated at the end of the study. The stools of the experimental rats were collected for estimating the gut microbiota.Results: Curcumae-treated group rats exposed reduction of the hepatic nodules in hepatic tissue. Curcumae significantly (P< 0.001) diminished the level of hepatic parameters and antioxidant parameters in the serum. Curcumae significantly (P< 0.001) suppressed the pro-inflammatory cytokines level, viz. interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), interleukin-2 (IL-2), interleukin-7 (IL-7) and augmented the level of interleukin-10 (IL-10) in the serum and hepatic tissue. Curcumae significantly (P< 0.001) suppressed inflammatory mediators including cyclooxygenase (COX-2), prostaglandin E2 (PGE2) and nuclear factor kappa B (NF-κB) in the serum and hepatic tissue. Furthermore, curcumae increased the gut microbial diversity and richness and decreased the relative abundance of genus Mucispirillum and Clostridium, respectively.Conclusion: Curcumae prevents HFD-induced inflammation during the hepatic carcinoma by modulating the oxidative stress, inflammatory reaction and gut microbiota.Keywords: curcumae, hepatic cellular carcinoma, gut microbiota, inflammation, antioxidant
