PRODUÇÃO DE AMILOGLUCOSIDASE UTILIZANDO COMO SUBSTRATO A PALMA FORRAGEIRA

Abstract

This paper aimed to evaluate the use of cactus pear as the main substrate for the production of amiloglucosidase by solid state fermentation with the aid of the filamentous fungus Aspergillus niger. The experiments were conducted at the Laboratory of Agro-industrial wastes at the State University of Southwest Bahia - UESB. The cactus pear was dried up to about 2% moisture. The variables in the fermentation process were water activity (0.891, 0.964, 0.976 and 0.983) and fermentation time (24 h, 48 h, 72 h, 96 h and 120 h). The results showed high enzyme production by the third day of fermentation, irrespective of water activity in all trials, after the fermentation time reduction of enzyme activity was observed, with the highest activity was 60.32 U/g in 72 hours and the water activity of 0.891. During the fermentation process the fungus synthesized and excreted the enzyme without the need for any other inducer addition to forage cactus and water, thus demonstrating the viability of using the palm as raw materials for bioprocesses.