Neutralizing Antibodies Induced by First-Generation gp41-Stabilized HIV-1 Envelope Trimers and Nanoparticles

Sonu Kumar; Xiaohe Lin; Timothy Ngo; Benjamin Shapero; Cindy Sou; Joel D. Allen; Jeffrey Copps; Lei Zhang; Gabriel Ozorowski; Linling He; Max Crispin; Andrew B. Ward; Ian A. Wilson; Jiang Zhu

doi:10.1128/mBio.00429-21

mBio (Jun 2021)

Neutralizing Antibodies Induced by First-Generation gp41-Stabilized HIV-1 Envelope Trimers and Nanoparticles

Sonu Kumar,
Xiaohe Lin,
Timothy Ngo,
Benjamin Shapero,
Cindy Sou,
Joel D. Allen,
Jeffrey Copps,
Lei Zhang,
Gabriel Ozorowski,
Linling He,
Max Crispin,
Andrew B. Ward,
Ian A. Wilson,
Jiang Zhu

Affiliations

Sonu Kumar: Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California, USA
Xiaohe Lin: Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California, USA
Timothy Ngo: Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California, USA
Benjamin Shapero: Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California, USA
Cindy Sou: Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California, USA
Joel D. Allen: School of Biological Sciences, University of Southampton, Southampton, United Kingdom
Jeffrey Copps: Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California, USA
Lei Zhang: Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California, USA
Gabriel Ozorowski: ORCiD; Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California, USA
Linling He: Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California, USA
Max Crispin: School of Biological Sciences, University of Southampton, Southampton, United Kingdom
Andrew B. Ward: ORCiD; Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California, USA
Ian A. Wilson: ORCiD; Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California, USA
Jiang Zhu: ORCiD; Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California, USA

DOI: https://doi.org/10.1128/mBio.00429-21
Journal volume & issue: Vol. 12, no. 3

Abstract

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Self-assembling protein nanoparticles (NPs) presenting BG505 envelope (Env) trimers can elicit tier 2 HIV-1-neutralizing antibody (NAb) responses more effectively than soluble trimers. In the present study, monoclonal NAbs were isolated from previously immunized mice and rabbits for structural and functional analyses, which revealed that potent mouse NAbs recognize the C3/V4 region and small NP-elicited rabbit NAbs primarily target known glycan holes on BG505 Env.

Published in mBio

ISSN: 2161-2129 (Print); 2150-7511 (Online)
Publisher: American Society for Microbiology
Country of publisher: United States
LCC subjects: Science: Microbiology
Website: https://journals.asm.org/journal/mbio

About the journal