mBio
(Jun 2021)
Neutralizing Antibodies Induced by First-Generation gp41-Stabilized HIV-1 Envelope Trimers and Nanoparticles
Sonu Kumar,
Xiaohe Lin,
Timothy Ngo,
Benjamin Shapero,
Cindy Sou,
Joel D. Allen,
Jeffrey Copps,
Lei Zhang,
Gabriel Ozorowski,
Linling He,
Max Crispin,
Andrew B. Ward,
Ian A. Wilson,
Jiang Zhu
Affiliations
Sonu Kumar
Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California, USA
Xiaohe Lin
Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California, USA
Timothy Ngo
Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California, USA
Benjamin Shapero
Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California, USA
Cindy Sou
Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California, USA
Joel D. Allen
School of Biological Sciences, University of Southampton, Southampton, United Kingdom
Jeffrey Copps
Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California, USA
Lei Zhang
Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California, USA
Gabriel Ozorowski
ORCiD Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California, USA
Linling He
Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California, USA
Max Crispin
School of Biological Sciences, University of Southampton, Southampton, United Kingdom
Andrew B. Ward
ORCiD Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California, USA
Ian A. Wilson
ORCiD Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California, USA
Jiang Zhu
ORCiD Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California, USA
DOI
https://doi.org/10.1128/mBio.00429-21
Journal volume & issue
Vol. 12,
no. 3
Abstract
Read online
Self-assembling protein nanoparticles (NPs) presenting BG505 envelope (Env) trimers can elicit tier 2 HIV-1-neutralizing antibody (NAb) responses more effectively than soluble trimers. In the present study, monoclonal NAbs were isolated from previously immunized mice and rabbits for structural and functional analyses, which revealed that potent mouse NAbs recognize the C3/V4 region and small NP-elicited rabbit NAbs primarily target known glycan holes on BG505 Env.
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