Multifocal imaging for precise, label-free tracking of fast biological processes in 3D

Jan N. Hansen; An Gong; Dagmar Wachten; René Pascal; Alex Turpin; Jan F. Jikeli; U. Benjamin Kaupp; Luis Alvarez

doi:10.1038/s41467-021-24768-4

Nature Communications (Jul 2021)

Multifocal imaging for precise, label-free tracking of fast biological processes in 3D

Jan N. Hansen,
An Gong,
Dagmar Wachten,
René Pascal,
Alex Turpin,
Jan F. Jikeli,
U. Benjamin Kaupp,
Luis Alvarez

Affiliations

Jan N. Hansen: Institute of Innate Immunity, Biophysical Imaging, Medical Faculty, University of Bonn
An Gong: Center of Advanced European Studies and Research (caesar), Molecular Sensory Systems
Dagmar Wachten: Institute of Innate Immunity, Biophysical Imaging, Medical Faculty, University of Bonn
René Pascal: Center of Advanced European Studies and Research (caesar), Molecular Sensory Systems
Alex Turpin: School of Computing Science, University of Glasgow
Jan F. Jikeli: Institute of Innate Immunity, Biophysical Imaging, Medical Faculty, University of Bonn
U. Benjamin Kaupp: Center of Advanced European Studies and Research (caesar), Molecular Sensory Systems
Luis Alvarez: Center of Advanced European Studies and Research (caesar), Molecular Sensory Systems

DOI: https://doi.org/10.1038/s41467-021-24768-4
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 15

Abstract

Read online

Multifocal imaging suffers from a number of limitations. Here the authors report an open-source 3D reconstruction algorithm to enable label-free tracking of spherical and filamentous structures which they use to characterise fluid flow and flagellar beating of human and sea urchin sperm.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal