Food and Agricultural Immunology (Jan 2018)

Development of an immunochromatographic assay for rapid detection of clorprenaline in pig urine

  • Wei Jiang,
  • Lu Zeng,
  • Liqiang Liu,
  • Shanshan Song,
  • Hua Kuang

DOI
https://doi.org/10.1080/09540105.2017.1411469
Journal volume & issue
Vol. 29, no. 1
pp. 536 – 547

Abstract

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A clorprenaline (CLP) monoclonal antibody, 4G1, raised against the CLP-hapten was successfully prepared for the development of indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and immunochromatographic assays for the detection of CLP in pig urine samples. The half maximal inhibitory concentration of the antibody was 0.435 ng/mL, and the limit of detection was 0.104 ng/mL. The linear range was 0.104–1.818 ng/mL, and cross-reactivity with the CLP analogue was <5%. In the spiked sample and recovery test, the recovery ranged from 81.8% to 100.5%, indicating that the ic-ELISA was suitable for CLP analysis in pig urine. The critical value of the immunochromatographic strip in pig urine was 10 ng/mL and could be used for semi-quantitative analysis of CLP. Thus, this immunochromatographic strip was suitable for quickly and sensitively detecting CLP residues in pig urine samples.

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