BioTechniques
(Mar 2009)
Flexible low-cost system for small animal aerosol inhalation exposure to drugs, proteins, inflammatory agents, and infectious agents
William G. Schroeder,
Laura M. Mitrescu,
Marcia L. Hart,
Ranjana Unnithan,
John M. Gilchrist,
Erin E. Smith,
Crystal Shanley,
Katharine M. Benedict,
Lauren Taraba,
John Volckens,
Randall J. Basaraba,
Alan R. Schenkel
Affiliations
William G. Schroeder
1Department of Microbiology, Immunology & Pathology, Colorado State University, Fort Collins, CO, USA
Laura M. Mitrescu
1Department of Microbiology, Immunology & Pathology, Colorado State University, Fort Collins, CO, USA
Marcia L. Hart
1Department of Microbiology, Immunology & Pathology, Colorado State University, Fort Collins, CO, USA
Ranjana Unnithan
1Department of Microbiology, Immunology & Pathology, Colorado State University, Fort Collins, CO, USA
John M. Gilchrist
1Department of Microbiology, Immunology & Pathology, Colorado State University, Fort Collins, CO, USA
Erin E. Smith
1Department of Microbiology, Immunology & Pathology, Colorado State University, Fort Collins, CO, USA
Crystal Shanley
1Department of Microbiology, Immunology & Pathology, Colorado State University, Fort Collins, CO, USA
Katharine M. Benedict
1Department of Microbiology, Immunology & Pathology, Colorado State University, Fort Collins, CO, USA
Lauren Taraba
1Department of Microbiology, Immunology & Pathology, Colorado State University, Fort Collins, CO, USA
John Volckens
2Department of Environmental Health and Radiological Sciences, Colorado State University, Fort Collins, CO, USA
Randall J. Basaraba
1Department of Microbiology, Immunology & Pathology, Colorado State University, Fort Collins, CO, USA
Alan R. Schenkel
1Department of Microbiology, Immunology & Pathology, Colorado State University, Fort Collins, CO, USA
DOI
https://doi.org/10.2144/000112895
Journal volume & issue
Vol. 46,
no. 3S
Abstract
Read online
The design for a simple, low-cost aerosol generation system for rodent inhalation studies is described here. This system is appropriate for low biohazard–level agents. In this study, two biosafety level 2 agents, Pasturella pneumotropica and Pseudomonas aeruginosa, were tested successfully. This system was also used to immunize mice and guinea pigs in ovalbumin-based models of pulmonary inflammation. This design is appropriate for studies with limited budgets and lower-level biosafety containment.
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