Culturable hydrocarbonoclastic marine bacterial isolates from Indonesian seawater in the Lombok Strait and Indian Ocean
Agung Dhamar Syakti,
Priyati Lestari,
Satya Simanora,
Lilik Kartika Sari,
Febrianti Lestari,
Fadliyah Idris,
Teguh Agustiadi,
Syafsir Akhlus,
Nuning Vita Hidayati,
Riyanti
Affiliations
Agung Dhamar Syakti
Center for Maritime Biosciences Studies–Institute for Sciences and Community Service, Jenderal Soedirman University, Kampus Karangwangkal, Jl. dr. Suparno, Purwokerto, 53123, Indonesia; Marine Science and Fisheries Faculty-Raja Ali Haji Maritime University, Jl. Politeknik Senggarang-Tanjungpinang, Riau Islands Province, 29100, Indonesia; Corresponding author.
Priyati Lestari
Fisheries and Marine Science Faculty-Jenderal Soedirman University, Kampus Karangwangkal, Jl. dr. Suparno, Purwokerto, 53123, Indonesia
Satya Simanora
Fisheries and Marine Science Faculty-Jenderal Soedirman University, Kampus Karangwangkal, Jl. dr. Suparno, Purwokerto, 53123, Indonesia
Lilik Kartika Sari
Fisheries and Marine Science Faculty-Jenderal Soedirman University, Kampus Karangwangkal, Jl. dr. Suparno, Purwokerto, 53123, Indonesia
Febrianti Lestari
Marine Science and Fisheries Faculty-Raja Ali Haji Maritime University, Jl. Politeknik Senggarang-Tanjungpinang, Riau Islands Province, 29100, Indonesia
Fadliyah Idris
Marine Science and Fisheries Faculty-Raja Ali Haji Maritime University, Jl. Politeknik Senggarang-Tanjungpinang, Riau Islands Province, 29100, Indonesia
Teguh Agustiadi
Institute for Marine Research and Observation (BPOL)–Ministry of Marine Affairs and Fisheries of the Republic of Indonesia, Jl. Baru Perancak, Negara Jembran-Bali, 82251
Syafsir Akhlus
Department of Chemistry, Sepuluh Nopember Institute of Technology, Surabaya, Indonesia
Nuning Vita Hidayati
Fisheries and Marine Science Faculty-Jenderal Soedirman University, Kampus Karangwangkal, Jl. dr. Suparno, Purwokerto, 53123, Indonesia
Riyanti
Fisheries and Marine Science Faculty-Jenderal Soedirman University, Kampus Karangwangkal, Jl. dr. Suparno, Purwokerto, 53123, Indonesia
Purpose: The study aims to isolate the culturable marine bacteria and to assess their potential as the bioremediation agent for petroleum hydrocarbons contamination in marine environment. Methods: Bacteria isolates were obtained by repetitive streaks to obtain purified bacteria on Zobell marine agar plates before further analysis and culture through direct visualization on agar plates. Identification were conducted using 16S rDNA sequence which are compared using NCBI BLAST and, combined with phenotypic and phylogenetic data. The potential use of the selected bacteria was tested by culturing them with two carbon sources i.e., glucose and crude oil. Result: Fifty-one culturable marine hydrocarbonoclastic bacteria were isolated from the Lombok Strait (LS-3, LS-13, LS-14, LS-15, LS-16 and LS-20) and Indian Ocean (IO-1, IO-6, IO-8, IO-19, IO-24 and IO-25). Twelve isolates were found to degrade crude oil efficiently at a >2% concentration and to grow with crude oil as their sole carbon and energy source. These 12 strains belong to the genus Bacillus, which is well known to produce surface active agents, and the oil displacement assay indicated the production of these agents by these strains. Within the genera Bacillus, five species (Bacillus flexus, B. methylotrophicus, B. aquimaris, B. horikoshii, and B. thioparans) were represented by the 12 identified strains. Conclusion: Selected strains from the Lombok Strait and Indian Ocean were capable of degrading crude oil (2% v/v) by 43.9–71.9% over 14 days. These results are important for marine bioremediation in Indonesia, which often faces risks of oil spill contamination and disaster.
