VAMP7 regulates constitutive membrane incorporation of the cold-activated channel TRPM8

Debapriya Ghosh; Silvia Pinto; Lydia Danglot; Ine Vandewauw; Andrei Segal; Nele Van Ranst; Melissa Benoit; Annelies Janssens; Rudi Vennekens; Pieter Vanden Berghe; Thierry Galli; Joris Vriens; Thomas Voets

doi:10.1038/ncomms10489

Nature Communications (Feb 2016)

VAMP7 regulates constitutive membrane incorporation of the cold-activated channel TRPM8

Debapriya Ghosh,
Silvia Pinto,
Lydia Danglot,
Ine Vandewauw,
Andrei Segal,
Nele Van Ranst,
Melissa Benoit,
Annelies Janssens,
Rudi Vennekens,
Pieter Vanden Berghe,
Thierry Galli,
Joris Vriens,
Thomas Voets

Affiliations

Debapriya Ghosh: Department of Cellular and Molecular Medicine, Laboratory of Ion Channel Research and TRP Channel Research Platform Leuven (TRPLe), University of Leuven
Silvia Pinto: Department of Cellular and Molecular Medicine, Laboratory of Ion Channel Research and TRP Channel Research Platform Leuven (TRPLe), University of Leuven
Lydia Danglot: Institut Jacques Monod, CNRS UMR 7592, University of Paris Diderot
Ine Vandewauw: Department of Cellular and Molecular Medicine, Laboratory of Ion Channel Research and TRP Channel Research Platform Leuven (TRPLe), University of Leuven
Andrei Segal: Department of Cellular and Molecular Medicine, Laboratory of Ion Channel Research and TRP Channel Research Platform Leuven (TRPLe), University of Leuven
Nele Van Ranst: Department of Cellular and Molecular Medicine, Laboratory of Ion Channel Research and TRP Channel Research Platform Leuven (TRPLe), University of Leuven
Melissa Benoit: Department of Cellular and Molecular Medicine, Laboratory of Ion Channel Research and TRP Channel Research Platform Leuven (TRPLe), University of Leuven
Annelies Janssens: Department of Cellular and Molecular Medicine, Laboratory of Ion Channel Research and TRP Channel Research Platform Leuven (TRPLe), University of Leuven
Rudi Vennekens: Department of Cellular and Molecular Medicine, Laboratory of Ion Channel Research and TRP Channel Research Platform Leuven (TRPLe), University of Leuven
Pieter Vanden Berghe: Department of Clinical and Experimental Medicine, Laboratory for Enteric NeuroScience, University of Leuven
Thierry Galli: Institut Jacques Monod, CNRS UMR 7592, University of Paris Diderot
Joris Vriens: Department of Cellular and Molecular Medicine, Laboratory of Ion Channel Research and TRP Channel Research Platform Leuven (TRPLe), University of Leuven
Thomas Voets: Department of Cellular and Molecular Medicine, Laboratory of Ion Channel Research and TRP Channel Research Platform Leuven (TRPLe), University of Leuven

DOI: https://doi.org/10.1038/ncomms10489
Journal volume & issue: Vol. 7, no. 1
pp. 1 – 15

Abstract

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The temperature-sensitive TRPM8 channel is essential for cold sensing and has been linked to pathological cold hypersensitivity. Here, the authors find TRPM8 insertion in the cell membrane is mediated by VAMP7 following atypical LAMP1-containing vesicle transport, and that loss of VAMP7 leads to reduced cold avoidance in vivo.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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