Nature Communications (Jul 2019)

A genetically encoded probe for imaging nascent and mature HA-tagged proteins in vivo

  • Ning Zhao,
  • Kouta Kamijo,
  • Philip D. Fox,
  • Haruka Oda,
  • Tatsuya Morisaki,
  • Yuko Sato,
  • Hiroshi Kimura,
  • Timothy J. Stasevich

DOI
https://doi.org/10.1038/s41467-019-10846-1
Journal volume & issue
Vol. 10, no. 1
pp. 1 – 16

Abstract

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Expression of genetically encoded antibodies for cell labelling is often limited by folding issues. Here, the authors engineer an anti-HA scFv antibody that works in the cellular environment and use it to track mRNA translation dynamics in living cells and to label proteins in live zebrafish embryos.