Functional Fermented Milk with Fruit Pulp Modulates the In Vitro Intestinal Microbiota
Tais Fernanda Borgonovi,
Mateus Kawata Salgaço,
Gislane Lelis Vilela de Oliveira,
Lucas Amoroso Lopes de Carvalho,
Daniel Guariz Pinheiro,
Svetoslav Dimitrov Todorov,
Kátia Sivieri,
Sabrina Neves Casarotti,
Ana Lúcia Barretto Penna
Affiliations
Tais Fernanda Borgonovi
Graduate Program in Food, Nutrition and Food Engineering, UNESP—São Paulo State University, São José do Rio Preto 15054-000, SP, Brazil
Mateus Kawata Salgaço
Graduate Program in Food, Nutrition and Food Engineering, UNESP—São Paulo State University, São José do Rio Preto 15054-000, SP, Brazil
Gislane Lelis Vilela de Oliveira
Graduate Program in Food, Nutrition and Food Engineering, UNESP—São Paulo State University, São José do Rio Preto 15054-000, SP, Brazil
Lucas Amoroso Lopes de Carvalho
Graduate Program in Agricultural and Livestock Microbiology, UNESP—São Paulo State University, Jaboticabal 14884-900, SP, Brazil
Daniel Guariz Pinheiro
Department of Agricultural, Livestock and Environmental Biotechnology, UNESP—São Paulo State University, Jaboticabal 14884-900, SP, Brazil
Svetoslav Dimitrov Todorov
ProBacLab, Laboratory of Food Microbiology, Department of Food Science and Experimental Nutrition, Faculty of Pharmaceutical Sciences, University of Sao Paulo, São Paulo 05508-000, SP, Brazil
Kátia Sivieri
Department of Food and Nutrition, School of Pharmaceutical Science, UNESP—Sao Paulo State University, Araraquara 14800-903, SP, Brazil
Sabrina Neves Casarotti
Faculty of Health Sciences, UFR—Federal University of Rondonópolis, Rondonópolis 78736-900, MT, Brazil
Ana Lúcia Barretto Penna
Institute of Biosciences, Humanities and Exact Sciences, Department of Food Engineering and Technology, UNESP—São Paulo State University, São José do Rio Preto 15054-000, SP, Brazil
The effect of putative probiotic fermented milk (FM) with buriti pulp (FMB) or passion fruit pulp (FMPF) or without fruit pulp (FMC) on the microbiota of healthy humans was evaluated. FM formulations were administered into a simulator of the human intestinal microbial ecosystem (SHIME®) to evaluate the viability of lactic acid bacteria (LAB), microbiota composition, presence of short-chain fatty acids (SCFA), and ammonium ions. The probiotic LAB viability in FM was affected by the addition of the fruit pulp. Phocaeicola was dominant in the FMPF and FMB samples; Bifidobacterium was related to FM formulations, while Alistipes was associated with FMPF and FMB, and Lactobacillus and Lacticaseibacillus were predominant in FMC. Trabulsiella was the central element in the FMC, while Mediterraneibacter was the central one in the FMPF and FMB networks. The FM formulations increased the acetic acid, and a remarkably high amount of propionic and butyric acids were detected in the FMB treatment. All FM formulations decreased the ammonium ions compared to the control; FMPF samples stood out for having lower amounts of ammonia. The probiotic FM with fruit pulp boosted the beneficial effects on the intestinal microbiota of healthy humans in addition to increasing SCFA in SHIME® and decreasing ammonium ions, which could be related to the presence of bioactive compounds.
