Induction of Anthocyanin Accumulation in Callus Culture of 'Karaerik' (Vitis vinifera L.) by Ultraviolet Irradiation Effect

Abstract

In this study, the effect of ultraviolet (UV) irradiation on the induction of anthocyanin production in callus cultures of 'Karaerik' grape cultivar was investigated. The most appropriate callus tissues were obtained from the leaves of the cuttings grown in in vitro plants. Gamborg B-5 medium containing 3 combinations of NAA (naphthalene acetic acid), and Kin (kinetin) was used. Callus tissues were subcultured two times with 21 days intervals. After the second subculture, 12 and 15 day old callus tissues were exposed to 254 nm UV-C light at 10 cm distance from the source for 10 and 15 min by opening covers of the petri dishes in the sterile cabin. After the treatment, callus tissues were incubated under dark conditions. Anthocyanin values were measured at 0, 24th, 48th and 72nd hours by spectrophotometer. It has been determined that UV radiation is effective for the induction of anthocyanin production in grape tissues. The highest anthocyanin production (196.74 μmol g-1 FW) was obtained from 12 day old calli grown in the first medium for a 72-hour incubation period after 10 min UV irradiation.

Keywords

• grapevine
• plant tissue culture
• elicitor
• secondary metabolite
• abiotic stress