Frontiers in Microbiology (Jun 2021)
Detection of Vibrio vulnificus in Seafood With a DNAzyme-Based Biosensor
- Shihui Fan,
- Shihui Fan,
- Shihui Fan,
- Chao Ma,
- Chao Ma,
- Chao Ma,
- Xiaopeng Tian,
- Xiaopeng Tian,
- Xiaopeng Tian,
- Xiaoyi Ma,
- Xiaoyi Ma,
- Xiaoyi Ma,
- Mingcan Qin,
- Mingcan Qin,
- Mingcan Qin,
- Hangjie Wu,
- Hangjie Wu,
- Hangjie Wu,
- Xueqing Tian,
- Xueqing Tian,
- Xueqing Tian,
- Jing Lu,
- Jing Lu,
- Jing Lu,
- Mingsheng Lyu,
- Mingsheng Lyu,
- Mingsheng Lyu,
- Shujun Wang,
- Shujun Wang,
- Shujun Wang
Affiliations
- Shihui Fan
- Jiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang, China
- Shihui Fan
- Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang, China
- Shihui Fan
- Jiangsu Marine Resources Development Research Institute, Lianyungang, China
- Chao Ma
- Jiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang, China
- Chao Ma
- Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang, China
- Chao Ma
- Jiangsu Marine Resources Development Research Institute, Lianyungang, China
- Xiaopeng Tian
- Jiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang, China
- Xiaopeng Tian
- Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang, China
- Xiaopeng Tian
- Jiangsu Marine Resources Development Research Institute, Lianyungang, China
- Xiaoyi Ma
- Jiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang, China
- Xiaoyi Ma
- Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang, China
- Xiaoyi Ma
- Jiangsu Marine Resources Development Research Institute, Lianyungang, China
- Mingcan Qin
- Jiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang, China
- Mingcan Qin
- Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang, China
- Mingcan Qin
- Jiangsu Marine Resources Development Research Institute, Lianyungang, China
- Hangjie Wu
- Jiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang, China
- Hangjie Wu
- Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang, China
- Hangjie Wu
- Jiangsu Marine Resources Development Research Institute, Lianyungang, China
- Xueqing Tian
- Jiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang, China
- Xueqing Tian
- Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang, China
- Xueqing Tian
- Jiangsu Marine Resources Development Research Institute, Lianyungang, China
- Jing Lu
- Jiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang, China
- Jing Lu
- Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang, China
- Jing Lu
- Jiangsu Marine Resources Development Research Institute, Lianyungang, China
- Mingsheng Lyu
- Jiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang, China
- Mingsheng Lyu
- Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang, China
- Mingsheng Lyu
- Jiangsu Marine Resources Development Research Institute, Lianyungang, China
- Shujun Wang
- Jiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang, China
- Shujun Wang
- Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang, China
- Shujun Wang
- Jiangsu Marine Resources Development Research Institute, Lianyungang, China
- DOI
- https://doi.org/10.3389/fmicb.2021.655845
- Journal volume & issue
-
Vol. 12
Abstract
Vibrio vulnificus is an important pathogenic bacterium that is often associated with seafood-borne illnesses. Therefore, to detect this pathogen in aquatic products, a DNAzyme-based fluorescent sensor was developed for the in vitro detection of V. vulnificus. After screening and mutation, a DNAzyme that we denominated “RFD-VV-M2” exhibited the highest activity, specificity, and sensitivity. The limit of detection was 2.2 × 103 CFU/ml, and results could be obtained within 5–10 min. Our findings suggested that the target of DNAzyme RFD-VV-M2 was a protein with a molecular weight between 50 and 100 kDa. The proposed biosensor exhibited an excellent capacity to detect marine products contaminated with V. vulnificus. Therefore, our study established a rapid, simple, sensitive, and highly specific detection method for V. vulnificus in aquatic products.
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