An optimized two-step chromatin immunoprecipitation protocol to quantify the associations of two separate proteins and their common target DNA

Abstract

Summary: Sequential chromatin immunoprecipitation (ChIP) is commonly used to investigate DNA-protein and protein-protein interactions to a specific genomic region. However, it can be tricky to achieve a robust and reproducible signal with sequential ChIP. Here, we provide an optimized two-step ChIP protocol to quantify the in vivo associates of multiple proteins with the same DNA regulatory element.For complete details on the use and execution of this protocol, please refer to He et al. (2020).

Keywords

• Molecular Biology
• Chromatin immunoprecipitation (ChIP)