Micropropagation of Catalpa bignonioides Walt. through tissue cultures

Abstract

The conditions used in the micropropagation of Catalpa bignonioides Walt. (Bignoniaceae) from callus tissue are described. The multiplication of shoots was best on Schenk and Hildebrandt (SH) solid medium supplemented with 0.5mg dm-3 indolil-3-acetic acid (IAA) and 2.0 mg dm-3 benzylaminopurine (BAP). Under these conditions, 11 shoots per culture could be developed within 4 weeks. To develop roots, the shoots were then transferred to basal SH medium without growth regulators. Rooted plantlets were obtained within 2-8 weeks. Regenerated plants were fully capable of further development in soil. Generally, ca 10 plants could be obtained from a fragment of callus within 10-12 weeks.

Keywords

• Catalpa bignonioides Walt.
• Bignoniaceae
• micropropagation
• tissue cultures
• rate of regeneration