The World Journal of Men's Health (Apr 2022)

CD4+/CD8+ Ratio and Growth Differentiation Factor 8 Levels in Peripheral Blood of Large Canine Males Are Useful Parameters to Build an Age Prediction Model

  • Han-Jun Lee,
  • Seok-Jin Hong,
  • Seung-Soo Kim,
  • Young-Yon Kwon,
  • Bong-Hwan Choi,
  • Kyung-Mi Choi,
  • Seo-Hyeong Sheen,
  • Myung-Jin Lee,
  • Sun-Young Hwang,
  • Kyoungwan Park,
  • Younghun Joo,
  • Hwayoung Song,
  • Cheol-Koo Lee

DOI
https://doi.org/10.5534/wjmh.210003
Journal volume & issue
Vol. 40, no. 2
pp. 316 – 329

Abstract

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Purpose: To build an age prediction model, we measured CD4+ and CD8+ cells, and humoral components in canine peripheral blood. Materials and Methods: Large Belgian Malinois (BGM) and German Shepherd Dog (GSD) breeds (n=27), aged from 1 to 12 years, were used for this study. Peripheral bloods were obtained by venepuncture, then plasma and peripheral blood mononuclear cells (PBMCs) were separated immediately. Six myokines, including interleukin (IL)-6, IL-8, IL-15, leukemia inhibitory factor (LIF), growth differentiation factor 8 (GDF8), and GDF11 were measured from plasma and CD4+/CD8+ T-lymphocytes ratio were measured from PBMC. These parameters were then tested with age prediction models to find the best fit model. Results: we found that the T-lymphocyte ratio (CD4+/CD8+) was significantly correlated with age (r=0.46, p=0.016). Among the six myokines, only GDF8 showed a significant correlation with age (r=0.52, p=0.005). Interestingly, these two markers showed better correlations in male dogs than females, and BGM breed than GSD. Using these two age biomarkers, we could obtain the best fit in a quadratic linear mixed model (r=0.77, p=3×10-6). Conclusions: Age prediction is a challenging task because of complication with biological age. Our quadratic linear mixed model using CD4+/CD8+ ratio and GDF8 level showed a meaningful age prediction.

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