Effects of Succinate-Based Antioxidant on in vitro Conversion of Methemoglobin in Oxyhemoglobin

Abstract

The purpose of the study — to determine the feasibility of using the succinate-based antioxidant for the in vitro reduction of excessive methemoglobin to oxyhemoglobin in blood.Materials and Methods. Blood sampling was performed in five healthy donors in microvettes containing EDTA during prophylactic examinations. NaNO2 solution was added to blood samples in vitro in order to yield methemoglobin (MetHb). The complex drug containing the following active ingredients: succinic acid, inosine, riboflavin, nicotinamide, was used as an antioxidant. The absorption spectrum of red cell suspensions with different drug content Dι (λι )exper was measured with 1 nm increments. The non-linear regression method was used to calculate concentrations of hemoglobin derivatives in suspensions.Results. In our experiments, when methemoglobin reacted with drug the optical density of peaks typical for oxyhemoglobin increased and the spectral peak of methemoglobin decreased. The greater the concentration of drug, the more was the incubation time, the more efficient was the process of reduction of MetHb to HbO2.Conclusion. We proved experimentally that while the baseline concentration of MetHb was an average of 91—93%, addition of drug decreased its concentration to 25—7%. Without drug, due to autoreduction, the concentration of MetHb decreases only to 84%. The revealed effect provide a potential for practical applications in critical illness, during the storage of donor blood, in blood transfusions, and under the action of physico-chemical factors on blood.

Keywords

• red blood cells
• methemoglobin
• oxyhemoglobin
• reduction
• succinic acid
• inosine
• riboflavin
• nicotinamide
• cytoflavin