Antimutagenic, Cytoprotective and Antioxidant Properties of <i>Ficus deltoidea</i> Aqueous Extract In Vitro
Theng Choon Ooi,
Farah Wahida Ibrahim,
Shakirah Ahmad,
Kok Meng Chan,
Lek Mun Leong,
Nihayah Mohammad,
Ee Ling Siew,
Nor Fadilah Rajab
Affiliations
Theng Choon Ooi
Center for Healthy Aging and Wellness, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur 50300, Malaysia
Farah Wahida Ibrahim
Center for Toxicology and Health Risk Studies, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur 50300, Malaysia
Shakirah Ahmad
Center for Healthy Aging and Wellness, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur 50300, Malaysia
Kok Meng Chan
Center for Toxicology and Health Risk Studies, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur 50300, Malaysia
Lek Mun Leong
Prima Nexus Sdn Bhd, Suite 8-1 Level 8, Menara CIMB, Jalan Stesen Sentral 2, Kuala Lumpur 50470, Malaysia
Nihayah Mohammad
Center for Toxicology and Health Risk Studies, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur 50300, Malaysia
Ee Ling Siew
ASASI Pintar Program, Pusat GENIUS@Pintar Negara, Universiti Kebangsaan Malaysia, Bangi 43600, Malaysia
Nor Fadilah Rajab
Center for Healthy Aging and Wellness, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur 50300, Malaysia
Ficus deltoidea var. deltoidea is used as traditional medicine for diabetes, inflammation, and nociception. However, the antimutagenic potential and cytoprotective effects of this plant remain unknown. In this study, the mutagenic and antimutagenic activities of F. deltoidea aqueous extract (FDD) on both Salmonella typhimurium TA 98 and TA 100 strains were assessed using Salmonella mutagenicity assay (Ames test). Then, the cytoprotective potential of FDD on menadione-induced oxidative stress was determined in a V79 mouse lung fibroblast cell line. The ferric-reducing antioxidant power (FRAP) assay was conducted to evaluate FDD antioxidant capacity. Results showed that FDD (up to 50 mg/mL) did not exhibit a mutagenic effect on either TA 98 or TA 100 strains. Notably, FDD decreased the revertant colony count induced by 2-aminoanthracene in both strains in the presence of metabolic activation (p p F. deltoidea may provide a chemopreventive effect on mutagenic and oxidative stress inducers.
