Bioelectricity Generation by <i>Corynebacterium glutamicum</i> with Redox-Hydrogel-Modified Carbon Electrode

Abstract

This work studied Gram-positive and weak electricigen Corynebacterium glutamicum for its ability to transfer electrons and to produce bioelectricity in microbial fuel cells (MFCs). The electrochemical and liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) results revealed that C. glutamicum had the potential to mediate electron transfer to an electrode by emitting its own extracellular electron shuttles such as flavins. To enhance the current collection from C. glutamicum, a carbon cloth anode was modified with ferrocene-branched chitosan hydrogel (redox-hydrogel). The maximum current density of the ferrocene-branched chitosan redox hydrogel anode with C. glutamicum was drastically increased to 120 µA cm−2 relative to a bare carbon cloth electrode with C. glutamicum (261 nA cm−2). The power density and polarization curves for the MFC operation with the redox-hydrogel-modified anode showed that C. glutamicum effectively generated bioelectricity by means of the redox-hydrogel anode. The results suggest that, in such an electro-fermentation process, ferrocene-branched chitosan hydrogel grafted onto an anode surface would also facilitate both electron transfer from C. glutamicum to the anode and bioelectricity generation.

Keywords

• <i>corynebacterium glutamicum</i>
• bioelectricity
• redox-hydrogel
• carbon cloth electrode