Data on expression of lipoxygenases-5 and -12 in the normal and acetaminophen-damaged liver
Maria Suciu,
Alexandra T. Gruia,
Dragos V. Nica,
Seyed M.R. Azghadi,
Ani A. Mic,
Felix A. Mic
Affiliations
Maria Suciu
Electron Microscopy Integrated Laboratory, National Institute for Research and Development of Isotopic and Molecular Technologies, 67-103 Donath St., 400293 Cluj-Napoca, Romania; Molecular Biology and Biotechnology Department, Faculty of Biology and Geology, Babeş-Bolyai University, Cluj 5-7 Clinicilor St., 400006 Napoca, Romania
Alexandra T. Gruia
Regional Center for Transplant Immunology, County Clinical Emergency Hospital Timisoara, 10 Iosif Bulbuca Blvd., 300736 Timisoara, Romania
Dragos V. Nica
Regional Center for Transplant Immunology, County Clinical Emergency Hospital Timisoara, 10 Iosif Bulbuca Blvd., 300736 Timisoara, Romania; Faculty of Animal Sciences and Biotechnologies, Banat׳s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania”, Timisoara, Romania; Corresponding author at: Regional Center for Transplant Immunology, County Clinical Emergency Hospital Timisoara, 10 Iosif Bulbuca Blvd., 300736 Timisoara, Romania. Tel. +40 0356433211; Mobile:+40 733712955.
Seyed M.R. Azghadi
Department of Functional Sciences, University of Medicine and Pharmacy “Victor Babes”, 2 Eftimie Murgu Sq., 300041 Timisoara, Romania
Ani A. Mic
Department of Functional Sciences, University of Medicine and Pharmacy “Victor Babes”, 2 Eftimie Murgu Sq., 300041 Timisoara, Romania
Felix A. Mic
Department of Functional Sciences, University of Medicine and Pharmacy “Victor Babes”, 2 Eftimie Murgu Sq., 300041 Timisoara, Romania
Here we present additional data on the expression of lipoxygenases -5 and -12 in the normal and acetaminophen-damaged liver, which are associated with our manuscript recently published in Chemico-Biological Interactions on lipid metabolism and eicosanoid signaling pathways involved in acetaminophen-induced liver damage in a mouse model (http://dx.doi.org/10.1016/j.cbi.2015.10.019 [1]). It has been demonstrated that the expression of lipoxygenase-5 and leukotriene formation are increased in the livers of rats with carbon tetrachloride (CCl4)-induced cirrhosis (http://dx.doi.org/10.1053/gast.2000.17831 [2]). In addition, the lipoxygenase-12 is known to be expressed in the resident macrophage population of the liver (http://dx.doi.org/10.1016/S0014-5793(99)00396-8 [3]).Mice were injected with acetaminophen, and at 48 h their livers were processed for immunohistochemistry with anti-mouse lipoxygenase-5 and -12 antibodies. At the same time point, the RNA was also extracted from the liver to assess the expression of lipoxygenase-5 and -12 genes via qPCR analysis. Our results show that lipoxygenase-5 expression, but not that of lipoxygenase-12, changes significantly in the acetominophen-damaged liver.
