Cattle wastewater treatment using green microalga Coelastrella sp. KNUA068 as a promising bioenergy feedstock with enhanced biodiesel quality

Abstract

Global water scarcity increased the demand for clean water, leading to attention on microalgae-based biological treatment for wastewater due to economic feasibility and sustainable biomass applications. This study isolated indigenous microalga Coelastrella sp. KNUA068 from a wastewater treatment plant, observed its admissible growth rate in diluted cattle wastewater (DCW), and used it for wastewater treatment analysis. The microalga showed high growth rates in indoor and outdoor cultivation with 100% DCW. In addition, the ammonia nitrogen and nitrate nitrogen removal rates of the microalga were 69.97 and 60.35%, respectively, in indoor cultivation, and 50.63 and 67.20%, respectively, in outdoor cultivation. Carotenoid content analysis revealed lutein as the highest productivity carotenoid, and zeaxanthin production was higher in outdoor cultivation. The biomass exhibited suitable biodiesel quality with a cetane number of 50.8 for high-quality biodiesel production. Coelastrella sp. KNUA068 demonstrates potential for bioenergy feedstock, carotenoid production, and wastewater treatment. HIGHLIGHTS The valuable cetane number of microalgal biomass was suitable for high-quality biodiesel production.; The results of 20 L cultivation were similar to laboratory conditions.; Selection of strain that survives in livestock wastewater, and high growth rate of this microalgal strain.; Availability under the condition that microalgal biomass can be utilized as a byproduct.; High nutrient removal in a short period of time.;

Keywords

• biodiesel quality
• bioenergy feedstock
• cattle wastewater treatment
• cetane number
• microalgal biomass