Investigation of Ethidium Bromide Interaction with <i>Yersinia</i> Bacterial Cells in Lyophilized/Rehydrated Preparations

Abstract

Investigated was ethidium bromide interaction with the cells of avirulent Y. pestis and Y. pseudotuberculosis strains in lyophilized/rehydrated preparations. The population heterogeneity regarding particle size and concentration of nucleic acid was identified by flow cytofluorimetry and registration of total fluorescence intensity. Shown was the correlation between concentration of colony-forming units (CFU) and fluorescence increase after supplementing suspension with cetyltrimethylammoniumbromide. This approach was suggested as the express method to estimate viable cell count in lyophilized/rehydrated preparations and to control the process of preparing and storage of collection strains, dry live vaccine and probiotic preparations of gramnegative bacteria.

Keywords

• <i>yersinia pestis</i>
• <i>yersinia pseudotuberculosis</i>
• ethidium bromide
• fluorescence
• viability
• cytometry