Communications Chemistry (Sep 2022)

A genetically encoded BRET-based SARS-CoV-2 Mpro protease activity sensor

  • Anupriya M. Geethakumari,
  • Wesam S. Ahmed,
  • Saad Rasool,
  • Asma Fatima,
  • S. M. Nasir Uddin,
  • Mustapha Aouida,
  • Kabir H. Biswas

DOI
https://doi.org/10.1038/s42004-022-00731-2
Journal volume & issue
Vol. 5, no. 1
pp. 1 – 13

Abstract

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The main protease (Mpro) of SARS-CoV-2 is an important target for COVID-19 therapy. Here, a pair of genetically encoded BRET-based sensors for detecting Mpro activity are generated by sandwiching N-terminal autocleavage sites in between the mNeonGreen and NanoLuc proteins.